Magnetic Detection Method Using Dual-Label Particles
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Solution Overview
Problem
Existing optical detection methods for viruses and other objective substances suffer from reduced sensitivity due to non-specific adsorption of labeling particles, which complicates the detection of multiple types of objective substances.
Innovation Solution
A method utilizing magnetic particles and labeling particles that bind specifically to objective substances, allowing the conjugates to move by magnetic force, while using multiple kinds of labeling particles with different particle sizes or emission wavelengths to enable the detection of multiple objective substances.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If only labeling particles are used to bind to objective substance, then the detection method is simple, but non-specific adsorption causes noise that lowers detection sensitivity
Solution Approach 1:
The detection system is segmented into three distinct components: magnetic particles for magnetic manipulation, labeling particles for optical detection, and objective substances for target identification. This segmentation allows each component to perform its specific function independently, reducing non-specific adsorption noise while maintaining detection simplicity
Solution Approach 2:
Magnetic particles serve as an intermediary between the objective substance and the detection system. They enable magnetic manipulation and concentration of the conjugate without directly participating in the optical detection process, thereby reducing non-specific adsorption noise while maintaining detection sensitivity
2Measurement precision
If magnetic particles and labeling particles are used to bind to objective substance, then detection sensitivity is improved, but only one kind of objective substance can be detected
Solution Approach 1:
The detection system is designed with universal components that can detect multiple types of objective substances. Magnetic particles and labeling particles can bind to different objective substances through specific interactions, enabling a single system to perform multiple detection functions while maintaining high sensitivity
Solution Approach 2:
The system utilizes changes in particle parameters (size, magnetic properties, optical properties) to differentiate between multiple objective substances. By detecting variations in these parameters, the system can identify and distinguish between different types of objective substances simultaneously
3Adaptability or versatility
If multiple kinds of labeling particles with different particle sizes are used, then multiple objective substances can be detected, but the device complexity increases
Solution Approach 1:
The system uses labeling particles with different optical properties (analogous to color changes) to differentiate between multiple objective substances. These particles exhibit distinct optical signals that can be detected and distinguished, enabling multi-substance detection without requiring complex device modifications
Solution Approach 2:
The system adds the dimension of particle size variation to differentiate between multiple objective substances. By utilizing particles of different sizes with distinct optical and magnetic properties, the system can detect multiple substances simultaneously while using relatively simple detection equipment
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enhances detection sensitivity by minimizing noise from non-specifically adsorbed labeling particles and allows for the simultaneous detection of multiple types of objective substances.
Implementation Method 1
causing a conjugate of the objective substance, magnetic particles, and labeling particles to move by magnetic force
Implementation Method 2
capable of binding to another objective substance to which the first labeling particles do not bind, and the plurality of kinds of labeling particles satisfying at least one of a first condition that the labeling particles have mutually different particle sizes, or a second condition that the labeling particles generate signal lights upon being irradiated with light
Implementation Method 3
labeling particles (fluorescent particles or light scattering particles)
Data Source
AI summary
An object of the invention is to provide a method for detecting an objective substance, by which a plurality of kinds of objective substances can be detected by a single detection, in the detection of an objective substance from an analyte by utilizing magnetic force. In a case where an objective substance is detected by causing magnetic particles and labeling particles to bind to an objective substance and causing a conjugate thereof to move by magnetic force, the object is addressed by using a plurality of labeling particles including first labeling particles that bind to at least a first objective substance, and second labeling particles that bind to the first objective substance and bind to an objective substance to which the first labeling particles do not bind; and detecting an objective substance with a combination of bound labeling particles, the labeling particles satisfying at least one of a first condition that the particle sizes are different or a second condition that the signal lights generated upon light irradiation are different.