Multiplex Biomolecule Detection via Magnetic Encoding and Chemiluminescence
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Solution Overview
Problem
Current biomolecule detection methods, such as ELISA and light-initiated chemiluminescent technology, face challenges in multiplex detection and require repeated washing steps, making them time-consuming and complex, while existing multiplex detection methods like encoding microbead technology also require tedious washing processes, limiting their suitability for rapid on-site detection.
Innovation Solution
A detection particle system integrating encoding identification, magnetic separation, and light-initiated chemiluminescent detection functions, comprising a microcarrier with an encoding function and connected detection microparticles, allows for simultaneous multiplex detection of biomolecules without the need for washing, using a fluorescence microscope to collect and match signals for accurate analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If ELISA is used for biomolecule detection, then sensitivity and specificity are improved, but the detection process requires repeated separation and washing steps making it time-consuming and complex
Solution Approach 1:
The patent combines the detection particle with magnetic separation functionality and chemiluminescent signaling into a single integrated system. This merging of functions allows the detection particle to perform binding, separation, and signal generation simultaneously, eliminating the need for repeated washing steps while maintaining ELISA's sensitivity and specificity.
Solution Approach 2:
The patent replaces the mechanical washing and separation steps with magnetic separation. The magnetic detection particles can be separated and concentrated using a magnetic field, eliminating the need for repeated centrifugation and washing operations, thus significantly reducing detection time while maintaining measurement precision.
2Ease of operation
If light-initiated chemiluminescent technology is used, then washing steps are eliminated, but multiplex detection capability is lost
Solution Approach 1:
The detection particle is designed with multi-functionality: it incorporates magnetic separation capability, chemiluminescent signaling, and encoding features that enable multiplex detection. This universal design allows a single particle system to perform multiple functions simultaneously, including differential identification of multiple targets through encoding, while maintaining operational simplicity.
Solution Approach 2:
The patent uses encoding on the detection particles (such as fluorescent codes or magnetic properties) that can be differentially identified. This encoding system allows multiplex detection by enabling the differentiation of multiple targets through their unique codes, while the chemiluminescent signal provides the readout mechanism.
3Adaptability or versatility
If encoding microbead technology is used for multiplex detection, then multiple substances can be detected simultaneously, but repeated separation and washing steps are still required
Solution Approach 1:
The patent merges the encoding microbead functionality with magnetic separation capability and chemiluminescent signaling into a single integrated detection particle. This combination eliminates the need for separate washing and separation steps, reducing process complexity while maintaining multiplex detection capability through the encoding system.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables a highly sensitive, fast, and washing-free multiplex detection method, expanding the range of detectable biomolecules in a single reaction, simplifying experimental steps, and improving detection efficiency for proteins, nucleic acids, and small molecules.
Implementation Method 1
Light-initiated chemiluminescent technology employs the chemiluminescent signal generated by the transfer of singlet oxygen between two particles after light excitation to detect the analyte
Implementation Method 2
placing the mixture obtained in step (2) in a fluorescence microscope with a camera or photographic function to perform detection, and exciting the signal of the microcarrier with the encoding function and the light-initiated chemiluminescent signal
Data Source
AI summary
A detection particle suitable for multiplex detection of biomolecules, including a microcarrier with an encoding function, detection microparticles connected to the microcarrier, where the detection microparticle is suitable for light-initiated chemiluminescent detection. A multifunctional detection particle integrating encoding identification, magnetic separation, and light-initiated chemiluminescent detection functions was prepared. And an imaging detection and analysis device for the multifunctional detection particle was provided. The provided detection particle suitable for multiplex detection of biomolecules can realize a highly sensitive, fast, and washing-free multiplex detection method, which greatly increases the types of biomolecules that can be detected by a single reaction, and can remove unreacted signal labeled molecules without washing during the whole process, thus simplifying experimental steps, and improving detection efficiency. The method is suitable for the detection of proteins, nucleic acids, and small molecules.