Magnetic Microparticle Extraction via Field Control
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Solution Overview
Problem
Current automated extraction processes for analytes from complex sample materials are labor-intensive, require complex mechanical constructions, and have limitations in sample throughput and efficiency, particularly in the manipulation of magnetic or paramagnetic particles.
Innovation Solution
A liquid system that uses magnetic or paramagnetic microparticles with a functionalized surface, where a first mobile phase separates the particles from the sample material and a second mobile phase elutes the analyte, allowing for automated extraction and chromatographic analysis within a closed system, minimizing the need for mechanical complexity and consumables.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Extent of automation
If complex mechanical constructions are used for automated extraction, then automation extent is improved, but device complexity increases
Solution Approach 1:
The patent replaces complex mechanical manipulation systems with a magnetic field-based control system. Magnetic or paramagnetic particles are used as the extracting agent, and their movement, immobilization, and release are controlled by applying and removing magnetic fields, eliminating the need for complex mechanical valves, pumps, and positioning mechanisms typically required for automated extraction systems.
Solution Approach 2:
The system changes the physical state or position of the extracting agent by altering magnetic field parameters (presence, strength, direction) rather than using mechanical means. By controlling the magnetic field, the particles can be immobilized in specific zones for extraction and then released for regeneration, achieving automation through field parameter modulation instead of mechanical actuation.
2Manufacturing precision
If multiple washing and elution steps are performed, then manufacturing precision is improved, but loss of time increases
Solution Approach 1:
The patent enables continuous operation by regenerating the magnetic particles in situ within the same system. After elution, the particles are released from the extraction zone, washed with mobile phase in a continuous flow, and reused for the next extraction cycle without requiring manual intervention or system disassembly. This continuous circulation and regeneration process maintains high purification quality while minimizing cycle time.
Solution Approach 2:
The system performs preliminary immobilization of particles in specific zones before the actual extraction and washing steps. By pre-positioning the particles using magnetic fields and preparing the mobile phase flow paths in advance, the system optimizes the subsequent washing and elution steps, reducing the total time required while ensuring thorough purification.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enables efficient, automated extraction with high sample throughput and minimal consumables, achieving rapid cycle times and effective analyte purification, as demonstrated by the successful quantification of itraconazole in human plasma.
Implementation Method 1
a controllable means (96) which can be used to temporarily apply a local magnetic field to two or more consecutive sections (97) of the line (95)
Implementation Method 2
magnetic or paramagnetic microparticles (122)
Implementation Method 3
in one or more sections (97) of the extractor (90) the microparticles are immobilized by means of a magnetic field of a controllable means (96) and separated from the remaining sample material
Implementation Method 4
the second mobile phase (75) detaches the adsorbed analyte from the surface of the microparticles
Implementation Method 5
magnetic microparticles with a functionalized surface, where a first mobile phase separates the particles from the sample material
Data Source
Figure 1
Figure 2
Figure 3A~3B
AI summary
The invention concerns a device and a method for the manipulation of a liquid sample material in which magnetic microparticles are suspended whereby the microparticles have a functionalized surface and an analyte is bound to the surface. The sample material is introduced into a device with a liquid system through an injection device (50) and in a first mobile phase the sample material is carried to an extractor (90). In a section (97) of the extractor (90) the microparticles are immobilized by means of a magnetic field of a controllable means (96) and separated from the remaining sample material. By switching over of a switching unit (110) a second mobile phase (75) is carried to the extractor (90) and the second mobile phase (75) detaches the adsorbed analyte from the surface of the microparticles. The second mobile phase (75) with the dissolved analyte(s) can be analyzed by way of chromatographic separation (130) and subsequent detection (140).