Magnetic Microparticle Manipulation in High-Pressure Liquid Extraction
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Solution Overview
Problem
Current automated extraction processes for target analytes from complex sample materials are labor-intensive, require complex mechanical constructions, and have low sample throughput, limited series lengths, and consume large amounts of reagents and consumables.
Innovation Solution
A high-pressure liquid system with a device that manipulates magnetic or paramagnetic microparticles using a controllable magnetic field to immobilize and elute analytes, allowing for efficient separation and purification within a closed system, minimizing the need for manual handling and reagents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Extent of automation
If automated extraction processes are implemented using conventional mechanical systems, then labor intensity is reduced, but device complexity and mechanical construction requirements increase
Solution Approach 1:
The patent replaces conventional mechanical extraction systems with a magnetic field-based manipulation system. Magnetic microparticles are used to bind analytes, and a magnetic field is applied to separate the particles from the sample matrix, eliminating the need for complex mechanical separation devices while achieving full automation of the extraction process.
Solution Approach 2:
The patent changes the physical state and properties of the extraction system by using magnetic microparticles instead of conventional solid-phase extraction materials. The magnetic property parameter is introduced to enable field-based manipulation and separation, simplifying the overall system architecture while maintaining automation.
2Productivity
If conventional extraction methods are used, then sample throughput is limited, but process simplicity is maintained
Solution Approach 1:
The magnetic field-based separation system enables rapid processing of multiple samples by simply switching magnetic fields, allowing high sample throughput without requiring complex mechanical reconfiguration for each sample.
Solution Approach 2:
The patent employs periodic application and removal of magnetic fields to sequentially process multiple samples through the same extraction and separation system, enabling high throughput by rapidly cycling through samples without mechanical reconfiguration.
3Productivity
If magnetic microparticles are used for analyte binding, then extraction efficiency is improved, but separation complexity increases
Solution Approach 1:
The patent uses magnetic field application as a simple on/off mechanism to separate magnetic microparticles from the sample matrix, replacing complex mechanical separation devices while maintaining high extraction efficiency through magnetic binding.
4Loss of substance
If conventional extraction systems are used, then reagent consumption is high, but system simplicity is maintained
Solution Approach 1:
The patent introduces magnetic properties as a new parameter for analyte binding and separation, replacing conventional reagent-based extraction methods. This reduces reagent consumption while the magnetic field-based system remains relatively simple in design.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enables rapid and efficient extraction and purification of analytes with high sample throughput, reducing the requirement for complex mechanical components and reagents, and allowing for compact configuration and short cycle times.
Implementation Method 1
the controllable means (96) is capable of temporarily applying a local magnetic field to two or more consecutive sections (97) of the line (95)
Implementation Method 2
a magnetic field is applied to a first section (97a) of the line (95) located therein which is suitable for immobilizing the magnetic or paramagnetic microparticles (122) contained in the mobile phase entering through the line (95) on the nearest inner wall (93) of the line (95) facing the magnetic field
Implementation Method 3
contacting the liquid sample material (121) containing the analyte with microparticles (122) made of a magnetic or paramagnetic material with a functionalized surface (123) whereby the analyte adsorbs to the surface
Implementation Method 4
the second mobile phase (75) is suitable for detaching the adsorbed analyte from the surface of the microparticles (122)
Data Source
AI summary
Described are a device and a method for the manipulation of a liquid sample material in which magnetic microparticles are suspended whereby the microparticles have a functionalized surface and an analyte is bound to the surface. The sample material is introduced into a device with a liquid system through an injection device (50) and in a first mobile phase the sample material is carried to an extractor (90). In a section (97) of the extractor (90) the microparticles are immobilized by means of a magnetic field of a controllable means (96) and separated from the remaining sample material. By switching over of a switching unit (110) a second mobile phase (75) is carried to the extractor (90) and the second mobile phase (75) detaches the adsorbed analyte from the surface of the microparticles. The second mobile phase (75) with the dissolved analyte(s) can be analyzed by way of chromatographic separation (130) and subsequent detection (140).