Magnetic Nanoparticle Biomolecule Quantification

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Solution Overview

Problem

Current methods for measuring biomolecules using magnetic nanoparticles require additional processing steps and labor due to the need for optical or coloring agents for detection, increasing costs and complexity.

Innovation Solution

The method involves coating magnetic nanoparticles with bioprobes, allowing biomolecules to form particle clusters, and measuring magnetic properties such as relaxation time, saturated magnetization, or ac magnetic susceptibility to quantify biomolecules without the need for fluorescence labels or additional processing.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Difficulty of detecting and measuring

If optical or coloring agents are used for detection, then detection capability is improved, but processing steps and labor increase

Engineering Contradiction:

Improvedetection capabilityVSAvoidprocessing steps

Core Design Contradiction:

Difficulty of detecting and measuringVSDevice complexity

Solution Approach 1:

The patent extracts and eliminates the need for optical or coloring agents from the detection system. By using magnetic nanoparticles with inherent magnetic properties for detection, the method removes the requirement for additional optical labels, thereby reducing processing steps and labor while maintaining detection capability

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent replaces optical detection mechanisms with magnetic detection mechanisms. Instead of using optical agents that require complex imaging and processing, the invention uses magnetic properties of nanoparticles that can be detected through simpler magnetic measurement techniques, thus reducing device complexity

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Difficulty of detecting and measuring

If optical or coloring agents are used for detection, then detection capability is improved, but costs increase

Engineering Contradiction:

Improvedetection capabilityVSAvoidcosts

Core Design Contradiction:

Difficulty of detecting and measuringVSQuantity of substance

Solution Approach 1:

The patent employs magnetic nanoparticles as disposable detection elements that can be synthesized inexpensively and used directly without requiring expensive optical labels. The magnetic nanoparticles serve as both the functional element and the detection target, eliminating the need for costly fluorescent or colorimetric reagents

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

3Device complexity

If magnetic nanoparticles are used without optical agents, then processing steps are reduced, but detection precision may be affected

Engineering Contradiction:

Improveprocessing stepsVSAvoiddetection precision

Core Design Contradiction:

Device complexityVSMeasurement precision

Solution Approach 1:

The patent changes the detection parameter from optical properties to magnetic properties. By measuring magnetic characteristics such as magnetization or magnetic relaxation of the magnetic nanoparticles, the system achieves accurate quantification of biomolecules without requiring optical agents, thus maintaining detection precision while simplifying processing

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach reduces processing steps, labor, and costs by directly measuring magnetic properties of nanoparticles and clusters, enabling accurate quantification of biomolecules with enhanced sensitivity and reduced assay expenses.

Implementation Method 1

measuring the magnetic relaxation time of the solution to determine the amount of the biomolecules

Methodology Applied

Scientific EffectMagnetic relaxation:

Implementation Method 2

the biomolecules in the sample conjugate with the bioprobe molecules and the nanoparticles agglomerate to form particle clusters

Methodology Applied

Scientific EffectAgglomeration:

Implementation Method 3

measuring a saturated magnetization of the particle clusters to obtain the amount of the biomolecules

Methodology Applied

Scientific EffectSaturated magnetization: Magnetic Saturation

Implementation Method 4

measuring the ac magnetic susceptibility of the solution before and after adding a sample containing the biomolecules to be detected to the solution

Methodology Applied

Scientific EffectAc magnetic susceptibility:

Data Source

PatentUS7560289B2Methods of quantitatively measuring biomolecules

Publication Date: 2009.07.14 MAGQU

AI summary

The present invention is directed to methods for quantitatively measuring biomolecules by using magnetic nanoparticles. Through the use of the magnetic nanoparticles and the bioprobes coated to the magnetic nanoparticles, the biomolecules conjugated with the bioprobes result in the formation of particle clusters. By measuring the changes in magnetic properties resulting from the existence of the bio-targets, the amount of the bio-targets in a sample to be tested can be measured.