Magnetic Nanoparticle Bacteria Detection in Viscous Media
Solution Overview
Problem
Current methods for detecting food poisoning bacteria are slow, taking 3-5 days to produce diagnostic results, and struggle to effectively separate bacteria-bound particles from non-bound particles, requiring additional fluorescent particles for quantitative analysis.
Innovation Solution
A method using magnetic nanoparticles that capture bacteria through antigen-antibody interaction, enriched by magnetism, and separated in a highly viscous medium under a magnetic field, allowing for the distinct separation and quantitation of bacterium-complexed nanoparticles without additional equipment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If conventional culturing and biochemical assays are used to detect food poisoning bacteria, then diagnostic accuracy is maintained, but detection time is too long (3-5 days)
Solution Approach 1:
The patent replaces conventional biological culturing methods with magnetic separation technology. Magnetic nanoparticles functionalized with antibodies are used to capture target bacteria through magnetic field application, enabling separation and detection within minutes rather than days. This substitution of mechanical magnetic separation for biological culturing processes achieves rapid detection while maintaining accuracy.
2Measurement precision
If fluorescent nanoparticles are used for quantitative analysis of bacteria, then measurement capability is achieved, but additional particles and complexity are required
Solution Approach 1:
The patent employs magnetic nanoparticles that serve multiple functions simultaneously: they enable magnetic separation of bacteria-bound particles from non-bound particles, and they allow quantitative analysis through measurement of magnetic signal intensity. This multi-functional approach eliminates the need for separate fluorescent particles, simplifying the system while maintaining both separation efficiency and quantitative measurement capability.
3Reliability
If magnetic particles are used for bacterial separation, then separation capability is improved, but effective separation of bacteria-bound and non-bound particles becomes difficult
Solution Approach 1:
The patent introduces a highly viscous medium to change the physical parameters of the separation environment. In this viscous medium, bacteria-bound magnetic nanoparticles exhibit significantly reduced mobility compared to non-bound particles. When a magnetic field is applied, this viscosity difference amplifies the separation effect, allowing bacteria-bound particles to be effectively captured while non-bound particles remain mobile and can be washed away, achieving high separation efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and efficient separation and quantitation of bacteria-bound particles, reducing the time to obtain diagnostic results and eliminating the need for additional fluorescent particles, thus enhancing the speed and accuracy of food poisoning bacteria detection.
Implementation Method 1
enriching the magnetic nanoparticles from the sample
Implementation Method 2
passing the enriched magnetic nanoparticles through a highly viscous medium in presence of a magnetic field to separate the magnetic nanoparticles
Implementation Method 3
highly viscous medium
Implementation Method 4
magnetic nanoparticles capable of capturing bacteria into the sample and allowing the magnetic nanoparticles to capture bacteria
Data Source
AI summary
The present invention relates to a method for detecting food poisoning bacteria, and more particularly, to a method for rapidly and quantitatively isolating food poisoning bacteria contents which contaminate food and the like. The method according to the present invention is characterized by including the steps of: introducing magnetic nanoparticles which can bind to bacteria into a sample for measuring the bacteria so as to bind the magnetic nanoparticles to the bacteria; isolating the magnetic nanoparticles; passing the nanoparticles which are isolated by using magnetism through a solution having high viscosity so as to separate the magnetic nanoparticles to which bacteria are bound from magnetic nanoparticles to which no bacteria are bound; and quantifying the magnetic nanoparticles to which bacteria are bound.