Magnetic Sphere Coated Detection Agent for 25-Hydroxy Vitamin D
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Solution Overview
Problem
Current methods for detecting 25-hydroxyvitamin D suffer from poor stability of 25-hydroxyvitamin D antigen derivatives, leading to short storage life and stringent storage conditions, which limits their large-scale commercial application due to photosensitivity and susceptibility to oxidation.
Innovation Solution
A detection agent comprising a conjugate of 25-hydroxyvitamin D antigen derivative and a protein carrier coated on magnetic spheres, which enhances stability by restricting mobility and shielding from irradiation, improving binding specificity and extending the effective life span.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If 25-hydroxyvitamin D antigen derivative is used in detection, then detection accuracy is improved, but stability deteriorates due to photosensitivity and oxidation
Solution Approach 1:
The patent uses a magnetic sphere as an intermediary carrier to immobilize the 25-hydroxyvitamin D antigen derivative. The magnetic sphere provides a stable support structure that protects the antigen derivative from direct exposure to light and oxygen, thereby maintaining detection accuracy while improving stability. The conjugate formation between antigen derivative and magnetic sphere creates a protected microenvironment.
Solution Approach 2:
The patent creates a protected environment for the antigen derivative by coating it on magnetic spheres and formulating it in a conjugate structure. This effectively creates an inert microenvironment that shields the photosensitive and oxidation-prone antigen derivative from harmful external factors, resolving the contradiction between maintaining reactivity for detection and protecting from degradation.
2Measurement precision
If 25-hydroxyvitamin D antigen derivative is used, then detection specificity is improved, but storage life shortens due to poor stability
Solution Approach 1:
The magnetic sphere acts as a mediator that maintains the immunological activity and specificity of the antigen derivative while providing long-term stability. The conjugate structure preserves the antigen's binding characteristics necessary for specific detection while protecting it from degradation over time, thereby extending storage life without compromising specificity.
Solution Approach 2:
The patent creates a composite material system consisting of the magnetic sphere, conjugate, and antigen derivative. This composite structure combines the stability and protective properties of the magnetic sphere with the immunological specificity of the antigen derivative, achieving both long storage life and maintained detection specificity.
3Stability of the object's composition
If stringent storage conditions are applied, then stability is maintained, but ease of operation deteriorates due to complex storage requirements
Solution Approach 1:
The magnetic sphere-conjugate-antigen derivative system is designed to be self-protecting. The structural design inherently provides stability without requiring external protective measures such as special temperature control or shielding. The system serves itself by incorporating protective features into its basic structure, making it easy to store and handle while maintaining stability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution provides a stable and accurate detection method for 25-hydroxyvitamin D with improved stability and extended shelf life, enabling more reliable and efficient detection results comparable to LC-MS methods.
Implementation Method 1
magnetic spheres coated by the conjugate
Implementation Method 2
magnetic spheres coated by the conjugate formed by a 25-hydroxyvitamin D antigen derivative and a protein carrier
Data Source
Figure 1~2
Figure 3~4
AI summary
Provided are a detection agent for detecting hydroxy vitamin D, preparation method thereof, and use thereof in 25-hydroxy vitamin D immunological detection. The detection agent comprises a conjugate formed by a 25-hydroxy vitamin D antigen derivative and protein carrier, and magnetic spheres coated by the conjugate. Also provided is a 25-hydroxy vitamin D detection kit comprising the detection agent.