Maize 1DLIB1009 Breeding via Cytoplasmic Male Sterility

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Solution Overview

Problem

The development of new maize varieties is a time-consuming process that takes six to twelve years, and breeders aim to create stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved agronomic characteristics.

Innovation Solution

A novel maize variety, 1DLIB1009, is developed through careful breeding and selection, incorporating traits like male sterility, disease resistance, and improved yield, achieved through backcross conversion, genetic manipulation, and transformation, allowing for the creation of hybrid seeds with enhanced agronomic properties.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional maize breeding processes are used to develop new varieties with desirable traits, then the varieties achieve stability and high yielding characteristics, but the development process takes six to twelve years

Engineering Contradiction:
Improvevariety stabilityVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by using male sterile lines and cytoplasmic male sterility (CMS) systems to prevent self-pollination before breeding occurs. This ensures that cross-pollination is the only means of reproduction, thereby maintaining genetic purity and variety stability without requiring lengthy verification periods. The male sterility trait is introduced early in the breeding process to control pollination outcomes.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses cytoplasmic male sterility (CMS) as an intermediary mechanism to control pollination. The CMS trait acts as a mediator that prevents self-pollination while allowing controlled cross-pollination, thereby accelerating the breeding process. This intermediary system enables breeders to efficiently produce hybrid seeds with desired traits without waiting through multiple generations of stability verification.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Adaptability or versatility

If traditional breeding methods are used to combine desirable traits, then the varieties achieve agronomic soundness, but the process is time-consuming

Engineering Contradiction:
Improveagronomic adaptabilityVSAvoidbreeding development time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-selecting and pre-combining desirable traits in the parental lines before hybridization. The male sterile parent and male fertile parent are each developed with specific desirable traits already integrated, so that the F1 hybrid inherits a comprehensive set of agronomically sound characteristics in a single generation, rather than requiring multiple generations of selection.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent merges desirable traits from two different parental lines into a single F1 hybrid. The male sterile line contributes certain desirable traits while the male fertile line contributes complementary traits, and their combination in the hybrid produces agronomic soundness with enhanced adaptability to various environmental conditions.

Inventive Principle:
Principle #5Merging (Combining)

3Productivity

If inbred lines are used for hybrid production, then hybrid vigor is achieved, but self-pollination must be prevented

Engineering Contradiction:
Improvehybrid yieldVSAvoidpollination control mechanism
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent uses cytoplasmic male sterility (CMS) as an intermediary biological mechanism to automatically prevent self-pollination in the male sterile parent. This biological mediator eliminates the need for manual emasculation or other complex mechanical pollination control methods, thereby maintaining hybrid yield while reducing operational complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The male sterile parent performs self-service by inherently preventing its own self-pollination through the CMS trait. The plant's own cytoplasmic genetics prevent the formation of viable pollen, so the plant automatically ensures cross-pollination without requiring external intervention or complex control mechanisms.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS10813314B1Inbred corn line 1DLIB1009
Publication Date: 2020.10.27 AGRIGENETICS INC

AI summary

A novel maize variety designated 1DLIB1009 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1DLIB1009 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1DLIB1009 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1DLIB1009 or a locus conversion of 1DLIB1009 with another maize variety.