Maize 1PBLG71 Breeding via Cytoplasmic Male Sterility

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Solution Overview

Problem

The development of new maize varieties and hybrids is a time-consuming process, taking six to twelve years, and existing technologies face challenges in combining desirable traits such as disease resistance, drought tolerance, and high yield in a stable and agronomically sound manner.

Innovation Solution

A novel maize variety, 1PBLG71, is developed through careful breeding and selection, incorporating traits like male sterility, disease resistance, and drought tolerance, achieved through backcross conversion, genetic manipulation, and transformation, allowing for the creation of hybrid seeds with enhanced agronomic characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding methods are used to develop new maize varieties, then stable and agronomically sound varieties are achieved, but the development process takes six to twelve years

Engineering Contradiction:
Improvestability of maize varietyVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent utilizes male sterility traits and cytoplasmic factors as preliminary mechanisms to prevent self-pollination before hybridization occurs. This preliminary setup ensures that only desired cross-pollination takes place, streamlining the breeding process while maintaining variety stability and reducing development time.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent employs cytoplasmic male sterility factors as intermediaries to control pollination. These cytoplasmic factors act as mediators between parental lines, ensuring precise hybridization without requiring manual emasculation, thereby accelerating the breeding process while maintaining genetic stability.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If multiple desirable traits are combined in a single maize variety, then agronomic performance and yield are improved, but the complexity of the breeding program increases

Engineering Contradiction:
Improvemaize yieldVSAvoidbreeding program complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent merges multiple desirable traits including disease resistance, drought tolerance, and improved agronomic characteristics into single maize varieties through systematic hybridization programs. This combining approach achieves high productivity while managing breeding complexity through organized crossing schemes.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The patent develops maize varieties with multi-functionality, incorporating resistance to multiple diseases, tolerance to various abiotic stresses, and improved yield characteristics simultaneously. This universal approach allows single varieties to perform multiple functions, reducing the need for separate breeding programs for each trait.

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Reliability

If male sterility is introduced to prevent self-pollination and ensure hybrid seed production, then hybrid purity is improved, but additional genetic manipulation steps are required

Engineering Contradiction:
Improvehybrid seed purityVSAvoidgenetic manipulation process
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent extracts and utilizes cytoplasmic male sterility factors from specific maize lines to create male-sterile female parents. This extraction of the sterility trait eliminates the need for manual emasculation and ensures hybrid purity, while the genetic manipulation is confined to the initial development of sterile lines rather than ongoing breeding operations.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent employs self-sterilization mechanisms through cytoplasmic male sterility, where the plant itself prevents self-pollination without requiring external intervention. This self-service approach maintains hybrid purity automatically, reducing the need for continuous manual management and complex genetic manipulation in subsequent breeding steps.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS11641836B1Maize inbred 1PBLG71
Publication Date: 2023.05.09 PIONEER HI BREED INTERNATIONAL INC

AI summary

A novel maize variety designated 1PBLG71 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PBLG71 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PBLG71 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PBLG71 or a locus conversion of 1PBLG71 with another maize variety.