Maize Hybrid Breeding With CMS for Stable Stress Tolerance

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Solution Overview

Problem

Existing hybrid maize development methods struggle to combine desirable traits such as disease resistance, heat and drought tolerance, and improved yield while maintaining uniformity and stability in plant characteristics, which are crucial for commercial crop production.

Innovation Solution

The development of a novel maize hybrid variety X10T088, produced by crossing specific inbred varieties and incorporating genetic loci for traits like male sterility, disease resistance, and altered metabolism through backcrossing and transformation, along with cytoplasmically-inherited traits to enhance hybrid performance.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional plant breeding is used to combine desirable traits, then resistance to diseases and insects, resistance to heat and drought, and greater yield can be achieved, but uniformity of plant characteristics such as germination, stand establishment, growth rate, maturity, and plant and ear height may be compromised

Engineering Contradiction:
Improveresistance to diseases, heat, and droughtVSAvoiduniformity of plant characteristics
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent employs inbred lines that are genetically segmented and standardized, where each line has fixed, uniform characteristics. By crossing these standardized inbreds, the hybrid combines the advantages of both parents while maintaining uniformity through the controlled genetic segmentation of the parent lines.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent utilizes cytoplasmic male sterility (CMS) systems that change the reproductive parameters of the plant, creating male-sterile lines that cannot self-pollinate. This parameter change enables controlled cross-pollination and maintains uniformity in the hybrid by preventing selfing, while still allowing the combination of desirable traits from different parents.

Inventive Principle:
Principle #35Parameter changes

2Productivity

If mechanical harvesting is implemented, then productivity is improved, but uniformity of plant characteristics becomes more critical for consistent performance

Engineering Contradiction:
Improvemechanical harvesting efficiencyVSAvoiduniformity of plant characteristics
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent develops inbred lines with segmented, standardized genetic makeup that ensures uniform plant characteristics. This uniformity is essential for mechanical harvesting because it ensures consistent plant height, maturity timing, and ear positioning, allowing the harvesting machinery to operate efficiently across the entire field without encountering significant variability.

Inventive Principle:
Principle #1Segmentation

3Adaptability or versatility

If hybrid development combines multiple desirable traits, then adaptability and yield are improved, but the complexity of breeding programs increases

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent employs preliminary action by developing and stabilizing inbred lines before creating the final hybrid. These inbred lines are pre-selected and pre-characterized for specific traits, so that when they are crossed, the desired combination of traits is achieved more efficiently, reducing the overall complexity of the breeding program.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses cytoplasmic male sterility (CMS) as an intermediary mechanism to facilitate hybrid development. CMS simplifies the breeding process by providing a reliable method for controlled cross-pollination without requiring complex manual emasculation procedures, thereby reducing the operational complexity of combining multiple desirable traits.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS12478018B1Maize hybrid X10T088
Publication Date: 2025.11.25 PIONEER HI BREED INTERNATIONAL INC
  • US12478018B1 patent drawing

AI summary

A novel maize variety designated X10T088 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X10T088 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X10T088 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X10T088, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X10T088 are provided. Methods for producing maize varieties derived from maize variety X10T088 and methods of using maize variety X10T088 are disclosed.