Maize Hybrid Trait Introgression for Uniform Yield Stability

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Solution Overview

Problem

Existing hybrid maize development methods struggle to combine desirable traits such as disease resistance, heat and drought tolerance, and improved yield while maintaining uniformity and stability, which are crucial for commercial crop production.

Innovation Solution

A novel maize hybrid variety X00R821 is developed by crossing specific inbred varieties and introducing genetic loci for traits like male sterility, disease resistance, and altered metabolism through backcrossing and transformation, with cytoplasmic inheritance of traits like CMS to enhance hybrid performance.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional plant breeding is used to develop hybrid maize, then desirable traits such as disease resistance, heat and drought tolerance, and improved yield can be combined, but uniformity of plant characteristics and stability are difficult to maintain

Engineering Contradiction:
Improveresistance to diseases and insectsVSAvoiduniformity of plant characteristics
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent divides the breeding program into distinct segments: developing inbred lines with specific traits, crossing them to create F1 hybrids, and maintaining separate recurrent parent lines. This segmentation allows each component to be optimized independently while ensuring uniformity in the final hybrid through controlled crossbreeding protocols.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs preliminary inbreeding of parent lines to establish uniform, stable genetic backgrounds before creating the F1 hybrid. This preliminary action ensures that when the cross occurs, the resulting hybrid exhibits consistent and uniform plant characteristics while incorporating the desired resistance traits.

Inventive Principle:
Principle #10Preliminary action

2Productivity

If mechanical harvesting is implemented, then crop production efficiency is improved, but uniformity of plant characteristics such as germination, stand establishment, growth rate, and maturity becomes critical

Engineering Contradiction:
Improvecrop production efficiencyVSAvoiduniformity of maturity
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent modifies key growth parameters through selective breeding and controlled crossbreeding to ensure uniformity in germination timing, stand establishment, growth rate, and maturity. By adjusting these parameters genetically, the crop achieves uniform development that is essential for efficient mechanical harvesting while maintaining high productivity.

Inventive Principle:
Principle #35Parameter changes

3Adaptability or versatility

If multiple desirable traits are combined in a single hybrid, then hybrid performance is improved, but the complexity of breeding programs increases

Engineering Contradiction:
Improveheat and drought toleranceVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent merges multiple desirable traits including disease resistance, heat tolerance, drought tolerance, and improved yield into a single hybrid variety through systematic crossbreeding. By integrating these traits into one unified genetic package, the program achieves enhanced hybrid performance while managing breeding complexity through organized, phased development.

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The hybrid maize variety X00R821 exhibits improved resistance to diseases and insects, better agronomic quality, and enhanced yield stability, ensuring uniformity and adaptability to various growing conditions.

Implementation Method 1

A cytoplasmically-inherited trait has been introduced into the hybrid plant. Such cytoplasmically-inherited traits are passed to progeny through the female parent in a particular cross. An exemplary cytoplasmically-inherited trait is the male sterility trait. Cytoplasmic-male sterility (CMS) is a pollen abortion phenomenon determined by the interaction between the genes in the cytoplasm and the nucleus.

Methodology Applied
Scientific EffectCytoplasmic inheritance:

Implementation Method 2

Cytoplasmic-male sterility (CMS) is a pollen abortion phenomenon determined by the interaction between the genes in the cytoplasm and the nucleus. Alteration in the mitochondrial genome and the lack of restorer genes in the nucleus will lead to pollen abortion.

Methodology Applied
Scientific EffectPollen abortion:

Implementation Method 3

The genetic locus may be a naturally occurring maize gene introduced into the genome of a parent of the variety by backcrossing, a natural or induced mutation, or a transgene introduced through genetic transformation techniques.

Methodology Applied
Scientific EffectGenetic transformation:

Data Source

PatentUS12484511B1Maize hybrid X00R821
Publication Date: 2025.12.02 PIONEER HI BREED INTERNATIONAL INC
  • US12484511B1 patent drawing

AI summary

A novel maize variety designated X00R821 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X00R821 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X00R821 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X00R821, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X00R821 are provided. Methods for producing maize varieties derived from maize variety X00R821 and methods of using maize variety X00R821 are disclosed.