Maize Hybrid X00R812 for Stable Multi-Trait Breeding

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Solution Overview

Problem

Existing hybrid maize development methods struggle to combine desirable traits such as disease resistance, heat and drought tolerance, and improved yield while maintaining uniformity and stability, which are crucial for commercial crop production.

Innovation Solution

A novel maize hybrid variety X00R812 is developed by crossing inbred varieties and incorporating genetic loci for traits like male sterility, herbicide tolerance, and disease resistance through backcrossing and genetic transformation, ensuring uniformity and stability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional plant breeding methods are used to combine multiple desirable traits, then the number of traits (disease resistance, heat tolerance, drought tolerance, yield) can be increased, but the uniformity and stability of plant characteristics deteriorate

Engineering Contradiction:
Improvenumber of desirable traitsVSAvoiduniformity of plant characteristics
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent segments the breeding process into distinct generations (P1, P2, F1, F2, F3, etc.) with specific selection criteria for each generation. This structured segmentation allows systematic combination of multiple traits while maintaining control over uniformity at each stage, resolving the contradiction between trait diversity and compositional stability.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent performs preliminary actions by developing and characterizing parent lines (P1 and P2) with specific trait combinations before creating the hybrid. The parent lines are pre-selected and pre-characterized for uniformity, ensuring that the subsequent F1 hybrid inherits both multiple desirable traits and uniformity, thus resolving the contradiction between adaptability and stability.

Inventive Principle:
Principle #10Preliminary action

2Reliability

If multiple traits are combined in a single hybrid, then the overall crop performance and resistance improve, but the complexity of the breeding process increases

Engineering Contradiction:
Improvecrop performance and resistanceVSAvoidbreeding process complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The breeding process is divided into clearly defined generations (P1, P2, F1, F2, F3, BC1, BC2, etc.) with specific objectives for each generation. This segmentation simplifies the overall complexity by breaking down the multi-trait combination process into manageable stages, each with focused selection criteria, thereby resolving the contradiction between improved reliability and process complexity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Parent lines P1 and P2 are developed and characterized in advance with specific trait profiles before hybridization. This preliminary characterization reduces breeding complexity by pre-establishing the genetic foundation for multiple traits, allowing the F1 hybrid to inherit these traits systematically rather than requiring complex multi-generational selection for each trait individually.

Inventive Principle:
Principle #10Preliminary action

3Productivity

If inbred varieties are crossed to produce hybrids, then yield and vigor improve through heterosis, but the difficulty of maintaining genetic uniformity decreases

Engineering Contradiction:
Improveyield and vigorVSAvoidgenetic uniformity
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent develops and stabilizes parent inbred lines (P1 and P2) through multiple generations of selfing and selection before crossing. This preliminary inbreeding ensures high genetic uniformity within each parent line, which then allows the F1 hybrid to exhibit consistent heterosis across all plants. The uniform genetic background of parents ensures uniform hybrid expression, resolving the contradiction between productivity and manufacturing precision.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent creates multiple copies of the standardized F1 hybrid through controlled crossing of standardized P1 and P2 parents. Each F1 plant is a genetic copy of the hybrid genotype, ensuring uniformity in yield and vigor expression. This copying mechanism maintains genetic uniformity while preserving the heterotic advantage, resolving the contradiction between productivity and manufacturing precision.

Inventive Principle:
Principle #26Copying

Data Source

PatentUS12389851B1Maize hybrid X00R812
Publication Date: 2025.08.19 PIONEER HI BREED INTERNATIONAL INC
  • US12389851B1 patent drawing

AI summary

A novel maize variety designated X00R812 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X00R812 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X00R812 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X00R812, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X00R812 are provided. Methods for producing maize varieties derived from maize variety X00R812 and methods of using maize variety X00R812 are disclosed.