Maize Hybrid X08M626 Molecular Marker Selection

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Solution Overview

Problem

Traditional plant breeding methods face challenges in simultaneously achieving desirable traits such as disease resistance, drought tolerance, and uniformity in crop characteristics, which are essential for hybrid crops like maize, especially under mechanical harvesting conditions.

Innovation Solution

The development of a novel maize hybrid variety, X08M626, which is produced by crossing two inbred maize varieties and incorporates traits like male sterility, disease resistance, and drought tolerance through locus conversion and transformation techniques, ensuring uniformity and improved agronomic qualities.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional plant breeding methods are used to develop hybrid maize varieties, then multiple desirable traits such as disease resistance, drought tolerance, and uniformity can be combined, but the process is time-consuming and difficult to achieve simultaneous improvement of multiple traits

Engineering Contradiction:
Improvedesirable traits combinationVSAvoidbreeding process time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by using molecular markers to pre-identify and select parent lines that possess desired traits before crossing. This allows breeders to predict offspring characteristics in advance, significantly reducing the time required for trait combination compared to traditional trial-and-error breeding methods.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent implements feedback mechanisms through molecular marker-assisted selection, where genetic markers provide continuous information about the presence and combination of desired traits in breeding populations. This feedback enables real-time selection decisions, accelerating the breeding process while ensuring multiple traits are successfully combined.

Inventive Principle:
Principle #23Feedback

2Stability of the object's composition

If traditional breeding methods are used to achieve uniformity in plant characteristics, then consistency in germination, stand establishment, growth rate, and maturity can be improved, but the complexity of managing multiple traits simultaneously increases

Engineering Contradiction:
Improveplant characteristic uniformityVSAvoidbreeding program complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

Molecular markers provide objective feedback on the genetic composition of breeding materials, enabling precise tracking of uniformity for multiple traits simultaneously. This reduces the complexity of managing breeding programs by replacing subjective phenotypic assessment with objective molecular data.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent replaces traditional mechanical/phenotypic selection methods with molecular marker-based selection. This substitution simplifies the breeding process by using DNA analysis instead of time-consuming field observations and manual selection, reducing program complexity while improving uniformity.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Productivity

If mechanical harvesting is implemented, then harvesting efficiency is improved, but uniformity of plant characteristics such as maturity and plant height becomes critical and difficult to achieve with traditional breeding

Engineering Contradiction:
Improveharvesting efficiencyVSAvoidplant characteristic uniformity
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent uses preliminary molecular marker analysis to select parent lines with genetically determined uniform maturity and height characteristics. This preliminary genetic selection ensures that resulting hybrids will exhibit the uniformity required for mechanical harvesting, addressing the precision requirement before field trials.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS10285354B1Maize hybrid X08M626
Publication Date: 2019.05.14 PIONEER HI BREED INTERNATIONAL INC

AI summary

A novel maize variety designated X08M626 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X08M626 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X08M626 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X08M626, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X08M626 are provided. Methods for producing maize varieties derived from maize variety X08M626 and methods of using maize variety X08M626 are disclosed.