Maize Inbred 1PBRF25 for Accelerated Multi-Trait Breeding

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Solution Overview

Problem

The development of new maize varieties is a time-consuming process that requires years to combine desirable traits such as disease resistance, drought tolerance, and high yield, posing challenges in efficiently producing stable and agronomically sound maize varieties.

Innovation Solution

The development of a novel maize variety, 1PBRF25, which incorporates genetic traits through backcross conversion, genetic manipulation, and transformation, including cytoplasmic male sterility, to enhance traits like herbicide tolerance, insect resistance, and disease resistance, and can be propagated through tissue culture to maintain physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional plant breeding methods are used to develop new maize varieties, then desirable traits such as disease resistance, drought tolerance, and high yield can be combined, but the development process is time-consuming and takes six to twelve years

Engineering Contradiction:
Improvestability of maize varietyVSAvoiddevelopment time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by using doubled haploid technology to create inbred lines in a single generation rather than requiring multiple generations of selfing. This preliminary creation of homozygous lines accelerates the breeding process while maintaining the stability and reliability of the final variety, reducing development time from six to twelve years to a significantly shorter period.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent changes the temporal parameter of the breeding process by utilizing molecular markers and genomic selection to evaluate and select lines based on their genetic potential rather than waiting for phenotypic expression through multiple growing seasons. This parameter change in the selection methodology dramatically reduces the time required to develop stable, high-yielding varieties.

Inventive Principle:
Principle #35Parameter changes

2Adaptability or versatility

If multiple desirable traits are combined in a single variety through traditional breeding, then agronomic performance is improved, but the complexity of the breeding program increases

Engineering Contradiction:
Improveagronomic performanceVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the complex breeding program into distinct modular components: (1) creation of inbred lines via doubled haploids, (2) evaluation of inbred lines using molecular markers and genomic selection, (3) development of test crosses and hybrids, and (4) validation across environments. This segmentation allows each component to be optimized independently, reducing overall program complexity while maintaining high agronomic performance through the integration of multiple desirable traits.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS12433226B1Maize inbred 1PBRF25
Publication Date: 2025.10.07 PIONEER HI BREED INTERNATIONAL INC
  • US12433226B1 patent drawing

AI summary

A novel maize variety designated 1PBRF25 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PBRF25 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PBRF25 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PBRF25 or a locus conversion of 1PBRF25 with another maize variety.