Maize Inbred ID2516 Breeding Segmentation

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Solution Overview

Problem

Corn breeding faces challenges in producing high-yielding, agronomically sound plants that are uniformly adapted across the U.S. Corn Belt, as regional growing conditions and specific issues like Gray Leaf Spot infection, cool temperatures, and soil pH levels require region-specific traits, and existing methods struggle to introduce desired traits efficiently into inbred lines.

Innovation Solution

The development of maize inbred plant ID2516, which includes a mutant or transgenic gene conferring traits such as herbicide resistance, insect resistance, and abiotic stress tolerance, and a method for introducing desired heritable traits through crossing, selection, and backcrossing to produce hybrid seeds with specific characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If traditional breeding methods are used to develop maize inbreds, then uniformity and homozygosity are achieved, but vigor and seed yield are reduced

Engineering Contradiction:
ImprovehomozygosityVSAvoidseed yield
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The breeding program segments the development process into distinct phases: initial cross-pollination to create hybrid F1 generation with high vigor, followed by controlled self-pollination over multiple generations to achieve homozygosity in inbred lines. This segmentation allows each generation to serve its specific purpose without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies preliminary action by first creating the hybrid F1 generation with desired vigor and yield characteristics, then using this hybrid as the basis for developing subsequent inbred lines. The hybrid's superior traits are established before the homozygosity process begins, ensuring that the genetic foundation for high productivity is already in place.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If inbred lines are used for hybrid seed production, then reproducibility and uniformity are improved, but plant vigor is reduced

Engineering Contradiction:
ImproveuniformityVSAvoidplant vigor
Core Design Contradiction:
Stability of the object's compositionVSReliability

Solution Approach 1:

The breeding system dynamically transitions between heterozygous hybrid generation (for vigor) and homozygous inbred generation (for uniformity). The inbred lines serve as stable parental stocks that are periodically refreshed through new crosses, allowing the system to maintain both uniformity and vigor across different breeding cycles.

Inventive Principle:
Principle #15Dynamics

Solution Approach 2:

Desired vigor traits are established in the hybrid F1 generation before the inbred lines are fully developed. This preliminary establishment of vigor characteristics ensures that even as inbreds become highly homozygous, the hybrid offspring will inherit and express the desired vigor traits.

Inventive Principle:
Principle #10Preliminary action

3Adaptability or versatility

If multiple breeding objectives are pursued simultaneously, then trait diversity increases, but breeding program complexity increases

Engineering Contradiction:
Improvetrait diversityVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The breeding program segments trait incorporation into distinct phases: core traits (yield, vigor) are established in initial crosses, while secondary traits (disease resistance, stress tolerance) are introduced in subsequent backcrossing generations. This segmentation manages complexity by handling one set of traits at a time rather than all simultaneously.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs universal breeding methods including backcrossing, self-pollination, and selection that can be applied across multiple trait types. These multi-functional techniques allow the same procedural framework to handle diverse trait incorporation needs, reducing overall program complexity despite the variety of objectives.

Inventive Principle:
Principle #6Universality (Multi-functionality)

4Reliability

If regional adaptation is prioritized, then performance in specific areas improves, but overall adaptability across the Corn Belt decreases

Engineering Contradiction:
Improveregional performanceVSAvoidoverall adaptability
Core Design Contradiction:
ReliabilityVSAdaptability or versatility

Solution Approach 1:

The breeding program applies local quality by developing inbred lines with specific trait combinations tailored to different Corn Belt regions. Each regional variant incorporates traits appropriate for its environment (e.g., disease resistance for humid areas, drought tolerance for arid regions) while maintaining the core hybrid vigor foundation, allowing optimized performance in specific locations without sacrificing overall adaptability.

Inventive Principle:
Principle #3Local quality

Data Source

PatentUS9149014B2Variety corn line ID2516
Publication Date: 2015.10.06 SYNGENTA CROP PROTECITON AG

AI summary

The present invention provides an inbred corn line designated ID2516, methods for producing a corn plant by crossing plants of the inbred line ID2516 with plants of another corn plant. The invention further encompasses all parts of inbred corn line ID2516, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line ID2516, and plants produced according to these methods.