Maize Inbred PH24FS Breeding via Doubled Haploid Technology
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Solution Overview
Problem
The development of new maize varieties and hybrids is a time-consuming process that requires precise planning and efficient resource use, aiming to combine desirable traits such as disease resistance, drought tolerance, and high yield, while maintaining stability across various conditions and environments.
Innovation Solution
The introduction of the novel maize variety PH24FS, which involves crossing with another maize plant to introduce desired traits through backcross conversion, genetic manipulation, and transformation, including cytoplasmic or nuclear factors for male sterility, and the use of regenerable tissue cultures to produce plants with specific physiological and morphological characteristics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional breeding processes are used to develop new maize varieties, then desirable traits can be combined, but the process takes 6-12 years and is time-consuming
Solution Approach 1:
The patent applies preliminary action by using doubled haploid technology to create inbred lines in a single generation rather than requiring multiple generations of selfing. This preliminary creation of homozygous lines accelerates the breeding process while maintaining the stability and reliability of the final variety, reducing the overall breeding cycle from 6-12 years to a much shorter duration.
Solution Approach 2:
The patent replaces traditional mechanical breeding methods (repeated manual crossing and selection over multiple generations) with biotechnological methods including doubled haploid technology, molecular markers, and genomic selection. This substitution dramatically reduces the time required to develop stable, high-yielding maize varieties while maintaining breeding reliability.
2Productivity
If multiple breeding steps are performed to combine desirable traits, then yield and resistance improve, but the process complexity increases
Solution Approach 1:
The patent applies universality by developing a comprehensive breeding system that integrates multiple functions into a unified approach. The system combines doubled haploid technology, molecular marker assistance, genomic selection, and traditional phenotypic evaluation into a single integrated breeding platform. This multi-functional system efficiently combines desirable traits for high yield, disease resistance, and stress tolerance while managing process complexity through standardized protocols.
Solution Approach 2:
The patent implements feedback mechanisms through molecular marker-assisted selection and genomic evaluation that continuously monitor and adjust the breeding process. These feedback systems allow real-time assessment of trait progression and enable dynamic modification of breeding strategies, thereby achieving high productivity (yield and resistance) while controlling the complexity of the breeding process through data-driven decision making.
3Stability of the object's composition
If inbred lines are developed through traditional selfing, then homozygosity is achieved, but many generations are required
Solution Approach 1:
The patent replaces the traditional mechanical process of repeated selfing and selection with doubled haploid technology. This biotechnological method creates completely homozygous inbred lines in a single generation by doubling the haploid genome, eliminating the need for multiple generations of selfing. This substitution maintains the stability and homozygosity required for reliable variety development while dramatically reducing the time and generational steps involved.
Data Source
AI summary
A novel maize variety designated PH24FS and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH24FS with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH24FS through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH24FS or a locus conversion of PH24FS with another maize variety.