Maize Inbred PH24SA Breeding via Doubled Haploid Technology
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Solution Overview
Problem
Current maize breeding techniques face challenges in combining desirable traits such as disease resistance, drought tolerance, and uniformity, which are essential for efficient crop production and yield improvement.
Innovation Solution
Development of a novel maize variety, PH24SA, through a breeding process involving crossing inbred lines, doubled haploid production, and self-pollination to achieve homozygosity, along with potential genetic marker profiling for trait identification and locus conversions to introduce specific traits like herbicide tolerance or disease resistance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional plant breeding is used to combine desirable traits, then disease resistance and drought tolerance can be achieved, but the time required for breeding is extended and uniformity of plant characteristics is reduced
Solution Approach 1:
The patent employs doubled haploid technology to create completely homozygous lines in a single generation, performing the necessary genetic stabilization action in advance. This eliminates the need for multiple generations of self-pollination traditionally required, thus resolving the contradiction between achieving reliable trait combination and reducing breeding time.
Solution Approach 2:
The patent replaces the mechanical process of repeated self-pollination and selection with molecular marker-assisted selection. By using DNA markers to track desirable traits, the breeding process becomes more efficient and predictable, reducing both time and improving reliability of trait combination.
2Reliability
If traditional plant breeding is used to combine desirable traits, then disease resistance and drought tolerance can be achieved, but uniformity of plant characteristics is reduced
Solution Approach 1:
The patent creates completely homozygous doubled haploid lines that are genetically uniform from the start. This preliminary establishment of genetic uniformity ensures that all plants in a population will exhibit consistent characteristics, resolving the contradiction between achieving reliable disease resistance and maintaining uniformity.
Solution Approach 2:
The patent uses molecular marker feedback to monitor and select for both desirable traits and genetic uniformity simultaneously. By tracking marker profiles, breeders can ensure that selected plants maintain the desired level of homozygosity and uniformity while acquiring disease resistance traits.
3Productivity
If mechanical harvesting is used, then productivity is improved, but uniformity of plant characteristics becomes more critical and harder to achieve
Solution Approach 1:
The patent establishes complete homozygosity in the parental lines through doubled haploid technology before hybridization. This ensures that F1 hybrids will exhibit uniform characteristics necessary for mechanical harvesting, resolving the contradiction between high productivity and uniformity requirements.
Solution Approach 2:
The patent changes the genetic parameter of the parental lines from heterozygous or partially homozygous to completely homozygous through doubled haploid technology. This parameter change ensures uniform F1 hybrid performance, making the crop suitable for mechanical harvesting while maintaining high productivity.
Data Source
AI summary
A novel maize variety designated PH24SA and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH24SA with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH24SA through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH24SA or a locus conversion of PH24SA with another maize variety.