Maize Inbred PH252W Breeding via Molecular Markers

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Solution Overview

Problem

Current maize breeding techniques face challenges in combining desirable traits such as disease resistance, drought tolerance, and uniformity, which are essential for efficient crop production and mechanical harvesting.

Innovation Solution

Development of a novel maize variety, PH252W, with specific breeding processes and genetic modifications that enhance resistance to various stresses, diseases, and improve agronomic qualities like maturity and yield, through backcross conversion and transformation methods.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional plant breeding methods are used to combine desirable traits, then disease resistance and drought tolerance can be achieved, but the time required for breeding and the complexity of the process increases

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent utilizes pre-characterized inbred lines with known desirable traits (such as disease resistance and drought tolerance) that have been developed through prior breeding efforts. By selecting and crossing these pre-prepared inbred lines, the breeding process achieves reliable trait combination without requiring extensive time-consuming preliminary breeding work for each new variety.

Inventive Principle:
Principle #10Preliminary action

2Reliability

If traditional plant breeding methods are used to combine desirable traits, then disease resistance and drought tolerance can be achieved, but the uniformity of plant characteristics decreases

Engineering Contradiction:
Improvedisease resistanceVSAvoiduniformity of plant characteristics
Core Design Contradiction:
ReliabilityVSManufacturing precision

Solution Approach 1:

The patent divides the complex breeding process into distinct segments: developing standardized inbred lines with specific traits, characterizing them through molecular markers, and then systematically crossing them. This segmentation allows each component (disease resistance, drought tolerance, uniformity) to be optimized independently before combination, ensuring that the final hybrid maintains high uniformity while incorporating multiple desirable traits.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs molecular marker technology to monitor and control genetic parameters during breeding. By using DNA markers to track specific genes and traits, the breeding process can precisely control the inheritance and expression of desirable characteristics, ensuring uniformity in the resulting hybrid varieties while maintaining disease resistance and drought tolerance.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If mechanical harvesting is implemented, then productivity increases, but the requirement for uniformity of plant characteristics becomes more critical

Engineering Contradiction:
Improveharvest efficiencyVSAvoiduniformity of plant characteristics
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent uses molecular marker-assisted selection to precisely control key uniformity parameters such as plant height, maturity timing, and ear position. By monitoring these parameters at the genetic level during breeding, the resulting hybrid varieties exhibit the uniformity required for mechanical harvesting while maintaining high productivity, as the consistent plant architecture enables efficient mechanized operations.

Inventive Principle:
Principle #35Parameter changes

4Manufacturing precision

If backcross conversion and transformation methods are used to introgress traits, then the precision of trait introduction increases, but the device and process complexity increases

Engineering Contradiction:
Improvetrait introduction precisionVSAvoidbreeding process complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent employs molecular markers as intermediaries to facilitate precise trait introduction. These markers serve as detectable tags that allow breeders to track the inheritance of specific genes during backcrossing and transformation processes. The markers act as mediators between the complex genetic manipulation processes and the desired trait outcomes, enabling precise control without requiring direct observation of the complex molecular processes.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS9265221B1Maize inbred PH252W
Publication Date: 2016.02.23 PIONEER HI BREED INTERNATIONAL INC
  • US9265221B1 patent drawing

AI summary

A novel maize variety designated PH252W and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH252W with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH252W through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH252W or a locus conversion of PH252W with another maize variety.