Maize Inbred PH2DNR Breeding via Marker-Assisted Selection

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Solution Overview

Problem

The development of new maize varieties and hybrids is a time-consuming process that requires precise planning and efficient resource use, aiming to combine desirable traits such as disease resistance, drought tolerance, and high yield, while maintaining stability across different conditions and environments.

Innovation Solution

The introduction of the novel maize variety PH2DNR, which involves crossing with another maize plant to introduce traits like male sterility, herbicide tolerance, and disease resistance through backcross conversion and genetic transformation, along with processes for producing hybrid maize seeds and plants that ensure genetic homogeneity and stability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional maize breeding methods are used to combine desirable traits, then genetic diversity and trait combination are improved, but the breeding time and process duration increase significantly

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by creating inbred lines with predetermined desirable traits (disease resistance, drought tolerance, yield potential) before hybridization. The inbred PH2DNR is developed with specific traits already fixed through selective breeding, so that when crossed with other inbreds, the hybrid immediately expresses these pre-prepared traits without requiring additional breeding generations to accumulate them.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent utilizes parameter changes by transitioning from traditional slow genetic recombination to accelerated breeding methods including molecular marker-assisted selection and potential genetic transformation techniques. This changes the time parameter of trait combination from multiple generations (6-12 years) to fewer generations by directly selecting or introducing specific genetic markers associated with desirable traits.

Inventive Principle:
Principle #35Parameter changes

2Stability of the object's composition

If multiple generations of selective breeding are performed to achieve stability, then genetic homogeneity and variety stability are improved, but the resource consumption and breeding complexity increase

Engineering Contradiction:
Improvevariety stabilityVSAvoidbreeding process complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The patent applies feedback through molecular marker-assisted selection, where DNA markers linked to desirable traits are tracked through breeding generations. This molecular feedback system allows breeders to monitor genetic composition in real-time, confirming when sufficient homozygosity and stability are achieved without relying solely on phenotypic observation over multiple generations, thereby reducing breeding complexity.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent replaces the mechanical/systematic approach of traditional phenotypic selection with molecular-level detection methods. Instead of visually assessing and manually selecting plants over multiple generations, molecular markers provide direct genetic information about trait presence and homozygosity, substituting complex multi-generational phenotypic screening with simpler molecular analysis.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Productivity

If inbred lines with specific traits are developed through extensive breeding, then the agronomic performance and yield potential are improved, but the selfing generations and inbreeding time increase

Engineering Contradiction:
Improveyield potentialVSAvoidinbreeding time
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent applies parameter changes by using molecular marker technology to accelerate the inbreeding process. Instead of requiring 6-8 selfing generations to achieve sufficient homozygosity for high yield potential, molecular markers allow breeders to track allele fixation in real-time, potentially reducing the number of selfing generations needed while maintaining the same level of genetic purity and yield performance.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS9743612B1Maize inbred PH2DNR
Publication Date: 2017.08.29 PIONEER HI BREED INTERNATIONAL INC
  • US9743612B1 patent drawing

AI summary

A novel maize variety designated PH2DNR and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH2DNR with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH2DNR through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH2DNR or a locus conversion of PH2DNR with another maize variety.