Maize Inbred PH2G22 Breeding via Doubled Haploid Technology

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Solution Overview

Problem

The development of new maize varieties and hybrids is a time-consuming process, taking six to twelve years, and existing technologies face challenges in creating stable, high-yielding maize varieties with desirable traits such as disease resistance, drought tolerance, and improved agronomic characteristics.

Innovation Solution

A novel maize variety, PH2G22, is developed through careful breeding and selection, incorporating traits like male sterility, disease resistance, and drought tolerance, achieved through backcross conversion, genetic manipulation, and transformation, allowing for the creation of hybrid seeds with enhanced agronomic properties.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding methods are used to develop new maize varieties, then stable and high-yielding varieties can be achieved, but the development process takes six to twelve years

Engineering Contradiction:
Improveyield stabilityVSAvoidbreeding time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by using doubled haploid technology to create inbred lines in a single generation rather than requiring multiple generations of selfing. This preliminary creation of homozygous lines accelerates the breeding process while maintaining the stability and reliability of the resulting varieties.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent replaces the traditional mechanical/biological process of multi-generation selfing and selection with a biotechnological approach using doubled haploids. This substitution of the breeding mechanism dramatically reduces the time required to develop stable varieties from six to twelve years to just a few years.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Adaptability or versatility

If conventional breeding techniques are used, then maize varieties with desirable traits can be developed, but the process is time-consuming and inefficient

Engineering Contradiction:
Improvetrait incorporationVSAvoidbreeding efficiency
Core Design Contradiction:
Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent uses preliminary action by pre-establishing inbred lines through doubled haploid technology before the actual breeding program. This allows the inbred lines to be ready for immediate use in hybrid development, significantly improving breeding efficiency and trait incorporation speed.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent changes the fundamental parameter of generation time by using doubled haploid technology that creates homozygous lines in one generation rather than requiring multiple generations of selfing. This parameter change transforms the breeding process from a slow, multi-year endeavor to a rapid, efficient process.

Inventive Principle:
Principle #35Parameter changes

3Stability of the object's composition

If traditional inbreeding methods are used to create homozygous lines, then genetic stability is achieved, but the time required for line development is excessive

Engineering Contradiction:
Improvegenetic homogeneityVSAvoidline development time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent replaces the traditional multi-generation selfing process with a biotechnological doubled haploid system. This substitution achieves genetic homogeneity and stability in a single generation, dramatically reducing line development time while maintaining the genetic purity and stability required for commercial varieties.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent uses copying by creating doubled haploid lines that are genetic copies of the parent material but in a homozygous state. This copying process achieves the desired genetic stability and homogeneity much faster than traditional inbreeding methods, as the homozygosity is established in one generation rather than requiring multiple generations of selfing.

Inventive Principle:
Principle #26Copying

Data Source

PatentUS9826704B1Maize inbred PH2G22
Publication Date: 2017.11.28 PIONEER HI BREED INTERNATIONAL INC
  • US9826704B1 patent drawing

AI summary

A novel maize variety designated PH2G22 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH2G22 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH2G22 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH2G22 or a locus conversion of PH2G22 with another maize variety.