Maize Inbred PH2RTF Breeding via Molecular Markers

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Solution Overview

Problem

The development of new maize varieties is a time-consuming process that takes six to twelve years, and there is a continuous need for stable, high-yielding maize varieties with improved traits such as disease resistance, drought tolerance, and better agronomic characteristics.

Innovation Solution

A novel maize variety, PH2RTF, is developed through careful breeding and selection, incorporating traits like male sterility, disease resistance, and drought tolerance, achieved through backcross conversion, genetic manipulation, and transformation, allowing for the creation of hybrid seeds with enhanced agronomic properties.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional maize breeding methods are used to develop new varieties with improved traits, then disease resistance and yield stability are improved, but the breeding process takes six to twelve years which is too time-consuming

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-establishing a comprehensive set of molecular markers and genetic maps for the maize inbred line PH2RTF before breeding operations. This preparatory genetic characterization enables rapid trait identification and selection in subsequent breeding cycles, significantly reducing the time required to develop new varieties with improved disease resistance while maintaining breeding reliability

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If conventional breeding processes are followed to create stable maize varieties, then yield stability across environments is improved, but the process requires multiple generations of field testing which extends development time

Engineering Contradiction:
Improveyield stabilityVSAvoiddevelopment time
Core Design Contradiction:
Stability of the object's compositionVSDuration of action of moving object

Solution Approach 1:

The patent implements feedback mechanisms through the use of molecular markers that provide real-time genetic information about trait inheritance and expression. This feedback enables breeders to make informed selection decisions at each generation, accelerating the stabilization of yield characteristics across environments without requiring the full traditional timeline of multi-year field testing

Inventive Principle:
Principle #23Feedback

3Reliability

If traditional variety development processes are used, then agronomic soundness is improved, but the process from first cross to finished seed delivery takes six to twelve years

Engineering Contradiction:
Improveagronomic soundnessVSAvoidbreeding efficiency
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent replaces traditional mechanical/phenotypic selection methods with molecular marker-based selection systems. By substituting visual field evaluation with DNA-based genetic analysis, the breeding process achieves faster and more accurate selection for agronomic traits, significantly improving breeding efficiency while maintaining the reliability of agronomic soundness in developed varieties

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS10165752B1Maize inbred PH2RTF
Publication Date: 2019.01.01 PIONEER HI BREED INTERNATIONAL INC

AI summary

A novel maize variety designated PH2RTF and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH2RTF with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH2RTF through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH2RTF or a locus conversion of PH2RTF with another maize variety.