Maize Inbred PH2RVY Breeding via Doubled Haploid Technology
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Solution Overview
Problem
The development of new maize varieties and hybrids is a time-consuming process, taking six to twelve years, and existing technologies face challenges in creating stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved agronomic characteristics.
Innovation Solution
A novel maize variety, PH2RVY, is developed through careful breeding and selection, incorporating traits like male sterility, disease resistance, and drought tolerance, achieved through backcross conversion, genetic manipulation, and transformation, allowing for the creation of hybrid seeds with enhanced agronomic properties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional breeding processes are used to develop new maize varieties, then stable and high-yielding varieties can be obtained, but the development time is excessively long (6-12 years)
Solution Approach 1:
The patent applies preliminary action by using doubled haploid technology to create inbred lines in a single generation rather than requiring multiple generations of selfing. This preliminary creation of homozygous lines accelerates the breeding process while maintaining the stability and reliability of the resulting varieties.
Solution Approach 2:
The patent uses molecular markers and DNA profiling to create genetic copies and identify specific traits without requiring physical replication through multiple breeding generations. This allows rapid selection and verification of desirable traits while reducing the time needed to establish stable varieties.
2Productivity
If traditional breeding processes are used to develop new maize varieties, then agronomically sound varieties can be obtained, but the process is time-consuming and inefficient
Solution Approach 1:
The patent replaces traditional mechanical breeding operations (multiple generations of selfing and selection) with molecular biology techniques including DNA markers, molecular profiling, and doubled haploid technology. This substitution dramatically increases breeding efficiency and reduces the time required to develop new varieties.
Solution Approach 2:
The patent changes the temporal parameters of the breeding process by using accelerated methods such as doubled haploids that produce homozygous lines in one generation rather than requiring multiple generations. This parameter change in breeding cycle time maintains productivity while significantly reducing the overall development time.
3Adaptability or versatility
If conventional breeding methods are used, then maize varieties with desirable traits can be developed, but the complexity and effort required is excessive
Solution Approach 1:
The patent segments the complex breeding process into distinct modular steps: molecular marker selection, doubled haploid generation, trait verification through molecular profiling, and final variety development. This segmentation reduces the overall complexity by breaking down the breeding process into manageable, sequential operations that can be efficiently performed.
Solution Approach 2:
The patent introduces molecular markers and DNA profiling as intermediary tools that simplify the selection and verification of desirable traits. These intermediaries allow breeders to identify and select for specific traits without requiring complex multi-generational testing, thereby reducing the overall complexity of the breeding process while maintaining trait diversity.
Data Source
AI summary
A novel maize variety designated PH2RVY and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH2RVY with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH2RVY through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH2RVY or a locus conversion of PH2RVY with another maize variety.