Maize Inbred PH4CBV Breeding via Doubled Haploid Technology
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Solution Overview
Problem
The development of new maize varieties and hybrids is a time-consuming process, requiring six to twelve years from the initial cross to delivering finished seed to farmers, and existing technologies face challenges in combining desirable traits such as disease resistance, drought tolerance, and high yield in a stable and agronomically sound manner.
Innovation Solution
A novel maize variety, PH4CBV, is developed through careful breeding and selection, incorporating traits like male sterility, disease resistance, and drought tolerance via backcross conversion and genetic transformation, allowing for the creation of stable, high-yielding hybrids with improved agronomic characteristics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional breeding methods are used to develop new maize varieties, then the variety can be developed with desired traits, but the process takes six to twelve years which is time-consuming
Solution Approach 1:
The patent applies preliminary action by using doubled haploid technology to create inbred lines in a single generation rather than requiring multiple generations of selfing. The process involves creating haploid plants from pollen cells and then doubling their chromosomes to produce homozygous inbred lines immediately, skipping the gradual inbreeding process that would normally take several years.
Solution Approach 2:
The patent replaces the natural biological process of repeated self-pollination and selection with an artificial mechanical system - in vitro fertilization and tissue culture techniques. By using controlled pollination of emasculated plants and regenerating plants from tissue culture, the breeding process is accelerated from years to months while maintaining genetic stability.
2Adaptability or versatility
If multiple desirable traits are combined in a single variety, then the variety becomes more comprehensive, but the breeding complexity increases
Solution Approach 1:
The patent applies segmentation by developing separate inbred lines, each carrying specific desirable traits (such as disease resistance, drought tolerance, or yield characteristics), and then combining them through hybridization. This modular approach allows complex trait combinations to be achieved by assembling pre-developed genetic components rather than breeding all traits simultaneously.
Solution Approach 2:
The patent uses inbred lines as intermediary components that can be systematically combined to create hybrids with desired traits. These inbred lines serve as building blocks that simplify the breeding process by pre-establishing specific genetic combinations before final hybridization, reducing the overall complexity of achieving multiple trait combinations.
3Stability of the object's composition
If inbred lines are developed through traditional selfing, then genetic homozygosity is achieved, but the process requires multiple generations and careful selection
Solution Approach 1:
The patent replaces the natural biological process of repeated self-pollination with an artificial system involving in vitro pollination and tissue culture. By manually emasculating plants, collecting pollen, and regenerating plants from tissue culture, the process achieves genetic homozygosity in a single generation rather than requiring multiple generations of selfing, dramatically improving productivity.
Solution Approach 2:
The patent uses tissue culture techniques to create copies of plants from small tissue samples or cells. This allows rapid multiplication of inbred lines and recovery of homozygous genotypes without requiring the plants to go through multiple generations of selfing, thereby achieving both genetic stability and high productivity.
Data Source
AI summary
A novel maize variety designated PH4CBV and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH4CBV with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH4CBV through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH4CBV or a locus conversion of PH4CBV with another maize variety.