Maize Inbred PH4CTK Breeding via Male Sterility

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Solution Overview

Problem

The development of new maize varieties and hybrids is a time-consuming process, requiring six to twelve years from the first cross to the delivery of finished seed, and existing technologies face challenges in combining desirable traits such as disease resistance, drought tolerance, and high yield in a stable and agronomically sound manner.

Innovation Solution

A novel maize variety, PH4CTK, is developed through careful breeding and selection, incorporating traits like male sterility, disease resistance, and drought tolerance, achieved through backcross conversion, genetic manipulation, and transformation, allowing for the creation of hybrid seeds with enhanced agronomic characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding processes are used to develop new maize varieties, then desirable traits such as disease resistance, drought tolerance, and high yield can be combined, but the process takes six to twelve years and is time-consuming

Engineering Contradiction:
Improvestability of desirable traitsVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by using male sterility systems and predetermined breeding designs to prepare the genetic material and breeding structure in advance. This allows the actual crossing and trait combination to proceed more efficiently without the need for time-consuming manual emasculation and selection processes that traditionally extend breeding timelines.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent introduces male sterility as an intermediary mechanism to facilitate controlled crosses. By using cytoplasmic male sterility (CMS) systems and restorer genes as mediators, the breeding process achieves precise control over pollen flow and hybridization, enabling faster development of stable varieties with desired traits while reducing the time required for manual intervention and selection.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If multiple desirable traits are combined in a single variety, then agronomic performance and resistance are improved, but the complexity of the breeding program increases

Engineering Contradiction:
Improveresistance to diseases and environmental stressesVSAvoidbreeding program complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the breeding program into distinct modular components: male sterile lines carrying specific disease resistance genes, restorer lines with drought tolerance traits, and maintainer lines. This modular approach allows each trait to be developed and validated independently before combination, reducing overall program complexity while achieving multiple desirable traits in the final hybrid variety.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent creates universal breeding components that can serve multiple functions. For example, male sterile lines are designed to simultaneously provide disease resistance, serve as female parents in hybrid production, and enable controlled pollination. This multi-functionality reduces the number of separate breeding programs needed and simplifies the overall complexity of developing varieties with multiple desirable traits.

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Productivity

If manual emasculation and cross-pollination methods are used, then hybrid seeds can be produced, but the process requires substantial labor and time

Engineering Contradiction:
Improvehybrid seed productionVSAvoidlabor requirement
Core Design Contradiction:
ProductivityVSEase of operation

Solution Approach 1:

The patent applies self-service by utilizing cytoplasmic male sterility to automatically prevent self-pollination and enable controlled cross-pollination without manual emasculation. The male sterile plants naturally produce no viable pollen, eliminating the need for labor-intensive detasseling operations. This self-service mechanism dramatically reduces labor requirements while maintaining high hybrid seed production efficiency.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent replaces the mechanical system of manual emasculation and pollination control with a biological system based on cytoplasmic male sterility and nuclear restorer genes. This substitution eliminates the need for physical removal of tassels and manual pollination procedures, significantly reducing labor requirements and simplifying the hybrid seed production process while maintaining or improving productivity.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS11470805B2Maize inbred PH4CTK
Publication Date: 2022.10.18 PIONEER HI BREED INTERNATIONAL INC

AI summary

A novel maize variety designated PH4CTK and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH4CTK with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH4CTK through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH4CTK or a locus conversion of PH4CTK with another maize variety.