Maize Marker-Assisted Selection for Ear Weight

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Solution Overview

Problem

Current crop yields are insufficient to meet global demands by 2050, and agricultural land is shrinking due to industrialization and habitat use, necessitating the development of superior maize crop varieties with increased yield, particularly in terms of ear weight and yield-related traits.

Innovation Solution

The use of marker-assisted selection methods to identify and select maize plants with increased ear weight and yield by detecting QTL alleles associated with these traits on chromosome 2, specifically using markers such as PHM13853, PHM16785, and PHM7964, and introgressing these alleles into breeding programs.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional breeding methods are used to select maize plants with increased yield, then yield improvement can be achieved, but the breeding process is time-consuming and slow due to the complex and environmentally influenced nature of yield traits

Engineering Contradiction:
Improvebreeding speedVSAvoidbreeding time
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

Molecular markers serve as intermediaries to indirectly detect the presence of QTL alleles associated with yield traits. Instead of directly measuring complex yield phenotypes influenced by environment, the patent uses marker molecules as mediators to identify plants carrying desirable genetic variants, significantly accelerating selection speed and reducing breeding time

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces traditional mechanical/phenotypic selection methods with molecular detection methods. Instead of visually assessing and manually selecting plants based on complex yield traits, DNA extraction and marker analysis automatically identify plants with desired QTL alleles, substituting biochemical analysis for traditional breeding mechanics

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Measurement precision

If environmental conditions are considered in yield evaluation, then more accurate phenotypic assessment can be achieved, but environmental factors mask the expression of yield-related genes making selection difficult

Engineering Contradiction:
Improveyield assessment accuracyVSAvoidgene expression detection
Core Design Contradiction:
Measurement precisionVSDifficulty of detecting and measuring

Solution Approach 1:

The patent extracts the genetic signal from the phenotypic expression by directly analyzing DNA markers associated with QTLs. This separates the genetic contribution from environmental influences, allowing precise identification of yield-related genes without the masking effect of environmental conditions on phenotypic expression

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent performs preliminary genetic analysis at the DNA level before phenotypic expression occurs. By detecting QTL alleles through molecular markers in early plant stages, the method identifies yield-potential plants before environmental factors can mask or modify their phenotypic expression, enabling earlier and more accurate selection

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS10316370B2Compositions and methods for selecting maize plants with increased ear weight and increased yield
Publication Date: 2019.06.11 CORTEVA AGRISCIENCE LLC

AI summary

Compositions and methods useful in identifying and selecting maize plants with increased ear weight and yield are provided herein. The methods use molecular genetic markers to identify and/or select maize plants with increased ear weight and increased yield or to identify and counter-select maize plants with decreased ear weight and decreased yield.