Maize PH1K8C Breeding via Molecular Marker Selection

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Solution Overview

Problem

Current maize breeding techniques face challenges in combining desirable traits such as disease resistance, drought tolerance, and uniformity, which are essential for efficient crop production and yield improvement.

Innovation Solution

Development of a novel maize variety, PH1K8C, through specific breeding methods that involve crossing, backcrossing, and transformation to introduce desired traits, ensuring genetic stability and uniformity, and utilizing genetic marker profiles for identification and locus conversions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional plant breeding methods are used to combine desirable traits, then disease resistance and drought tolerance can be improved, but the time required to develop new varieties and the complexity of the breeding process increase

Engineering Contradiction:
Improvedisease resistanceVSAvoidtime to develop new variety
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by using molecular marker technology to pre-identify and select parent lines with desired traits before crossing. This allows breeders to predict offspring characteristics in advance, reducing the time required for field testing and selection across multiple generations.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent replaces traditional mechanical/phenotypic selection methods with molecular marker-based genotypic selection. Instead of visually assessing and testing plants in the field over multiple seasons, breeders use DNA markers to directly identify individuals carrying desired traits, dramatically accelerating the breeding cycle.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If multiple desirable traits are combined in a single variety, then yield and stress tolerance improve, but the genetic complexity and difficulty of maintaining uniformity increase

Engineering Contradiction:
ImproveyieldVSAvoidgenetic complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent implements feedback through molecular marker analysis at each breeding stage. By continuously monitoring the genetic composition of breeding lines using specific DNA markers, breeders can verify the presence of desired traits and make informed decisions about which individuals to advance, ensuring uniformity even as multiple traits are combined.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent applies segmentation by breaking down the complex task of combining multiple traits into manageable genetic components. Each desired trait is tracked using specific molecular markers, allowing breeders to independently manage and combine different trait combinations in separate breeding lines before final integration.

Inventive Principle:
Principle #1Segmentation

3Stability of the object's composition

If backcrossing and transformation methods are used to introgress traits, then genetic stability and uniformity improve, but the manufacturing complexity and cost of variety development increase

Engineering Contradiction:
Improvegenetic stabilityVSAvoidease of variety development
Core Design Contradiction:
Stability of the object's compositionVSEase of manufacture

Solution Approach 1:

The patent replaces complex multi-generation backcrossing procedures with direct molecular marker-assisted selection. Instead of performing repeated crosses and field selections to stabilize traits, breeders use DNA markers to directly identify and select individuals with the desired stable genetic composition, simplifying the development process.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces molecular markers as intermediaries between the breeder and the genetic traits. These markers serve as reliable indicators that mediate the selection process, allowing breeders to indirectly but accurately select for complex trait combinations without needing to directly observe or test each trait manifestation.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS8686251B1Maize inbred PH1K8C
Publication Date: 2014.04.01 PIONEER HI BREED INTERNATIONAL INC
  • US8686251B1 patent drawing

AI summary

A novel maize variety designated PH1K8C and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH1K8C with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH1K8C through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH1K8C or a locus conversion of PH1K8C with another maize variety.