Maize PH1KV7 Breeding via Molecular Markers

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Solution Overview

Problem

Current maize breeding techniques face challenges in combining desirable traits such as disease resistance, drought tolerance, and uniformity, which are essential for efficient crop production and mechanical harvesting.

Innovation Solution

Development of the novel maize variety PH1KV7, which is homozygous and can be reproduced through self-pollination, combined with advanced breeding methods like backcrossing and transformation to introduce desired traits, resulting in a hybrid with improved agronomic qualities.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional plant breeding methods are used to combine desirable traits, then disease resistance and drought tolerance can be achieved, but the time required for breeding and the complexity of the breeding process increase

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by using molecular markers to identify and select plants with desired traits at early stages of breeding. This allows breeders to predict and select for disease resistance and drought tolerance before the plants actually express these traits phenotypically, significantly reducing the time required for trait verification and breeding cycles.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent replaces traditional mechanical/phenotypic selection methods with molecular marker-based selection. Instead of waiting for plants to express disease resistance or drought tolerance through actual disease exposure or environmental stress, the invention uses DNA markers to detect the presence of desired genetic traits, substituting a biochemical detection system for traditional observational methods.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If multiple desirable traits are combined in a single variety, then agronomic quality and yield improve, but the uniformity of plant characteristics decreases

Engineering Contradiction:
ImproveyieldVSAvoiduniformity
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent applies local quality by using different molecular markers to track and select for specific local traits within the overall breeding program. Each marker corresponds to a specific gene or genetic region responsible for a particular trait (disease resistance, drought tolerance, yield), allowing breeders to independently control and optimize each trait while maintaining overall uniformity through marker-assisted selection.

Inventive Principle:
Principle #3Local quality

3Productivity

If backcrossing and transformation methods are used to introduce desired traits, then trait introduction efficiency improves, but the device complexity and process complexity increase

Engineering Contradiction:
Improvetrait introduction efficiencyVSAvoidbreeding process complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent uses molecular markers as intermediaries between the breeder and the genetic traits. These markers serve as detectable proxies for the actual traits of interest, allowing breeders to track and select for desired genes without directly observing the complex phenotypic expressions. This intermediary system simplifies the breeding process by providing clear, objective selection criteria based on DNA presence rather than complex phenotypic evaluation.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS8604312B1Maize inbred PH1KV7
Publication Date: 2013.12.10 PIONEER HI BREED INTERNATIONAL INC
  • US8604312B1 patent drawing

AI summary

A novel maize variety designated PH1KV7 and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH1KV7 with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH1KV7 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH1KV7 or a locus conversion of PH1KV7 with another maize variety.