Maize PH253T Breeding via Marker-Assisted Selection

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Solution Overview

Problem

Current maize breeding techniques face challenges in combining desirable traits such as disease resistance, drought tolerance, and uniformity, which are essential for efficient crop production and mechanical harvesting.

Innovation Solution

Development of a novel maize variety, PH253T, with specific breeding processes and genetic modifications to enhance traits like abiotic stress tolerance, disease resistance, and uniformity, achieved through backcross conversion and transformation methods.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional plant breeding methods are used to combine desirable traits, then the breeding process can be completed using conventional techniques, but the ability to effectively combine multiple traits such as disease resistance, drought tolerance, and uniformity is limited

Engineering Contradiction:
Improveability to combine multiple desirable traitsVSAvoidbreeding efficiency
Core Design Contradiction:
Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent applies parameter changes by utilizing molecular marker technology to transform the breeding process from phenotypic selection to genotypic selection. This changes the selection parameter from observable traits to specific DNA markers, enabling simultaneous tracking and combination of multiple desirable traits (disease resistance, drought tolerance, uniformity) through marker-assisted selection, thereby resolving the limitation of traditional breeding in effectively combining multiple traits

Inventive Principle:
Principle #35Parameter changes

2Manufacturing precision

If traditional breeding methods are used, then the process is simpler and more straightforward, but the precision in selecting and combining specific genetic traits is insufficient

Engineering Contradiction:
Improveprecision in selecting and combining genetic traitsVSAvoidcomplexity of breeding process
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent replaces the mechanical/phenotypic selection system with a molecular/genotypic selection system. Instead of visually selecting plants based on observable traits (mechanical observation), the invention uses DNA marker analysis to directly identify and select plants with desired genetic compositions. This substitution dramatically improves precision in selecting and combining specific genetic traits, as molecular markers provide direct evidence of gene presence regardless of environmental influences on phenotypic expression

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Productivity

If conventional breeding techniques are employed, then the time to develop new varieties is extended, but the ability to rapidly introduce disease resistance and stress tolerance is reduced

Engineering Contradiction:
Improvespeed of variety developmentVSAvoiddisease resistance and stress tolerance
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent applies preliminary action by pre-identifying and characterizing molecular markers associated with desirable traits (disease resistance, drought tolerance) before actual breeding begins. These markers serve as predictive indicators, allowing breeders to select parent plants and progeny with the desired genetic makeup early in the breeding process. This preliminary molecular characterization accelerates variety development while ensuring reliable introduction of disease resistance and stress tolerance, as selection is based on actual genetic composition rather than waiting for phenotypic expression

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS9554532B1Maize inbred PH253T
Publication Date: 2017.01.31 PIONEER HI BREED INTERNATIONAL INC
  • US9554532B1 patent drawing

AI summary

A novel maize variety designated PH253T and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH253T with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH253T through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH253T or a locus conversion of PH253T with another maize variety.