Maize Inbred PHPCH Breeding for Disease Resistance and Yield

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Solution Overview

Problem

Current maize breeding techniques face challenges in combining desirable traits such as disease resistance, drought tolerance, and uniformity, particularly in achieving stable and high-yielding varieties with improved agronomic qualities.

Innovation Solution

Development of the novel maize variety PHPCH, which involves crossing and backcrossing to introduce specific traits, and utilizing genetic marker profiles for locus conversions and hybrid production, ensuring homozygosity and phenotypic stability for commercial hybrid seed production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional plant breeding is used to combine desirable traits, then disease resistance and drought tolerance can be improved, but uniformity of plant characteristics and yield stability may be compromised

Engineering Contradiction:
Improvedisease resistanceVSAvoiduniformity of plant characteristics
Core Design Contradiction:
ReliabilityVSStability of the object's composition

Solution Approach 1:

The breeding program segments the combination of desirable traits by using backcrossing to introduce specific disease resistance and drought tolerance genes into the PHPCH genetic background separately, then combines them through controlled crossing. This allows each trait to be independently optimized while maintaining overall uniformity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies parameter changes by modifying the genetic composition through systematic backcrossing (BC1, BC2, BC3 generations) to achieve homozygosity at target loci while maintaining heterosis. The inbreeding coefficient is increased from 0.5 in F1 to 0.625 in BC1, 0.6875 in BC2, and 0.75 in BC3, progressively enhancing uniformity while preserving desired traits.

Inventive Principle:
Principle #35Parameter changes

2Adaptability or versatility

If multiple desirable traits are combined in a single variety, then agronomic quality improves, but the complexity of breeding and achieving homozygosity increases

Engineering Contradiction:
Improveagronomic qualityVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The breeding program performs preliminary action by first developing the PHPCH inbred line with a stable genetic background and desired base traits, then systematically introducing additional desirable traits through backcrossing. This staged approach simplifies the overall process compared to attempting to combine all traits simultaneously.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent employs feedback through molecular marker-assisted selection and phenotypic evaluation at each backcross generation to monitor progress toward homozygosity and trait expression. This allows real-time adjustment of breeding decisions to optimize the combination of multiple traits while managing process complexity.

Inventive Principle:
Principle #23Feedback

3Manufacturing precision

If backcrossing is used to introduce specific traits, then trait precision improves, but the time and generations required for breeding increases

Engineering Contradiction:
Improvetrait introduction precisionVSAvoidbreeding duration
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

The patent replaces traditional mechanical phenotypic selection with molecular marker-assisted selection to identify and select plants with desired traits and appropriate levels of homozygosity. This substitution accelerates the backcrossing process by enabling early-generation selection without waiting for phenotypic expression, reducing the effective time required despite multiple generations.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS7842865B1Maize variety inbred PHPCH
Publication Date: 2010.11.30 PIONEER HI BREED INTERNATIONAL INC
  • US7842865B1 patent drawing

AI summary

A novel maize variety designated PHPCH and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PHPCH with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHPCH through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PHPCH or a locus conversion of PHPCH with another maize variety.