Maize Inbred Line Trait Introgression for Faster Stable Breeding

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Solution Overview

Problem

The development of new maize varieties is a time-consuming process that requires years to combine desirable traits such as disease resistance, drought tolerance, and high yield, posing challenges in efficiently producing stable and agronomically sound maize varieties.

Innovation Solution

The development of a novel maize variety, 1PRME66, with specific traits introduced through backcross conversion, genetic manipulation, and transformation, including cytoplasmic male sterility and heritable traits like herbicide tolerance and disease resistance, facilitated by crossing and tissue culture techniques.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding methods are used to combine desirable traits, then the maize variety achieves desired traits such as disease resistance and high yield, but the development process takes six to twelve years

Engineering Contradiction:
Improvestability of desired traitsVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by using molecular marker technology to identify and select plants with desired traits at early stages of breeding, rather than waiting for traditional phenotypic expression. This allows breeders to pre-select individuals carrying specific genes for disease resistance, yield, and other desirable characteristics, significantly accelerating the breeding process while maintaining trait stability.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent replaces traditional mechanical/phenotypic selection methods with molecular marker-based selection. Instead of visually assessing plant traits after they manifest, the invention uses DNA markers to detect the presence of desired genes at the molecular level, enabling earlier and more accurate selection of breeding candidates.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Adaptability or versatility

If multiple desirable traits are combined through traditional breeding, then the maize variety achieves comprehensive improvement, but the process complexity increases

Engineering Contradiction:
Improvenumber of desirable traitsVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by breaking down the complex breeding process into manageable components using molecular markers for each specific trait. Each desired trait (disease resistance, yield, drought tolerance) can be tracked and selected for independently through specific molecular markers, allowing breeders to systematically combine multiple traits without being overwhelmed by the overall complexity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent implements feedback mechanisms through molecular marker analysis that provides real-time information about the genetic composition of breeding plants. This feedback allows breeders to make informed decisions at each generation, tracking the accumulation of desired traits and adjusting breeding strategies accordingly, thereby managing program complexity through systematic monitoring and control.

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS12514199B1Maize inbred 1PRME66
Publication Date: 2026.01.06 PIONEER HI BREED INTERNATIONAL INC
  • US12514199B1 patent drawing

AI summary

A novel maize variety designated 1PRME66 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PRME66 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PRME66 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PRME66 or a locus conversion of 1PRME66 with another maize variety.