Hybrid Maize X13B640 Locus Conversion for Trait Stability

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Solution Overview

Problem

The challenge in maize breeding is to combine desirable traits such as resistance to diseases and insects, heat, and drought tolerance with improved yield and agronomic quality, while maintaining uniformity and stability in hybrid crops.

Innovation Solution

The development of the hybrid maize variety X13B640, produced by crossing proprietary Pioneer Hi-Bred International inbred varieties, incorporates transgenes for insect and herbicide tolerance, with locus conversions that enhance traits like maturity, yield, and disease resistance, allowing for the introduction of specific desired traits through locus conversion and transformation processes.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional plant breeding is used to combine desirable traits, then resistance to diseases and insects, heat and drought tolerance can be improved, but the time to develop new varieties and maintaining uniformity becomes challenging

Engineering Contradiction:
Improveresistance to diseases and insects, heat and drought toleranceVSAvoidtime to develop new varieties
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs locus conversion technology to directly modify specific genetic loci in the maize variety, changing the genetic parameters precisely rather than through traditional time-consuming breeding methods. This allows rapid introduction of desired traits such as disease resistance and stress tolerance by converting specific loci with targeted genetic modifications.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces traditional mechanical breeding methods (crossing, selection, and recombination over multiple generations) with modern biotechnological methods including locus conversion and genetic transformation. This substitution enables direct genetic modification to achieve desired traits without the time delays inherent in traditional breeding cycles.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If multiple desirable traits are combined in a single hybrid, then yield and agronomic quality improve, but maintaining uniformity of plant characteristics becomes more difficult

Engineering Contradiction:
Improveyield and agronomic qualityVSAvoiduniformity of plant characteristics
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent applies local quality by making specific, targeted modifications at particular genetic loci rather than broad genomic changes. Each locus conversion addresses a specific trait (e.g., disease resistance at one locus, maturity at another) while leaving the rest of the genome unchanged, thereby maintaining overall uniformity while incorporating multiple desirable traits.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent segments the complex task of combining multiple traits into separate, manageable locus conversions. Each desired trait is introduced through independent locus conversion events at specific genetic locations, allowing precise control over which traits are combined and how they interact, thereby maintaining uniformity while achieving high productivity.

Inventive Principle:
Principle #1Segmentation

3Adaptability or versatility

If locus conversion and transformation processes are used to introduce specific traits, then desired traits can be precisely introduced, but the complexity of the breeding process increases

Engineering Contradiction:
Improveintroduction of specific desired traitsVSAvoidcomplexity of breeding process
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent uses locus conversion technology as an intermediary method between traditional breeding and direct genetic engineering. This intermediary approach allows for precise trait introduction through controlled locus-specific modifications, bridging the gap between conventional breeding simplicity and genetic engineering precision while managing process complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent employs preliminary action by first identifying and characterizing target loci before performing conversions. Molecular markers and genetic mapping are used beforehand to locate desired traits, and conversion strategies are planned in advance, which simplifies the actual execution process and reduces overall complexity despite the advanced technology involved.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS8822788B1Maize variety hybrid X13B640
Publication Date: 2014.09.02 PIONEER HI BREED INTERNATIONAL INC
  • US8822788B1 patent drawing

AI summary

A novel maize variety designated X13B640 and seed, plants and plant parts thereof, produced by crossing Pioneer Hi-Bred International, Inc. proprietary inbred maize varieties. Methods for producing a maize plant that comprises crossing hybrid maize variety X13B640 with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13B640 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X13B640, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13B640. This invention further relates to methods for producing maize varieties derived from maize variety X13B640.