Hybrid Maize X13B656 Locus Conversion for Trait Introgression

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Solution Overview

Problem

The challenge in maize breeding is to combine desirable traits such as resistance to diseases and insects, heat, and drought tolerance with improved yield and agronomic quality, while maintaining uniformity and stability in hybrid crops.

Innovation Solution

The development of the hybrid maize variety X13B656, produced by crossing proprietary Pioneer Hi-Bred International maize inbred varieties, which incorporates locus conversions and transformation to introduce specific traits, ensuring genetic integrity and enhanced performance.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional plant breeding methods are used to combine desirable traits, then the process is simple and maintains genetic uniformity, but the ability to introduce specific resistance traits and enhance performance is limited

Engineering Contradiction:
Improveability to introduce specific traitsVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent uses locus conversion as an intermediary technique to transfer specific resistance traits between inbred lines. This involves crossing the target inbred line with a donor line containing the desired trait, selecting progeny with the trait, and backcrossing to recover the original genetic background while retaining the introduced resistance gene. This mediator approach enables precise trait introduction while maintaining overall genetic uniformity.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The breeding process is segmented into distinct phases: initial crossing to introduce the trait, selection phase to identify progeny with the desired resistance, and backcrossing phases to recover the original genetic background. This segmentation allows systematic management of complexity while achieving both trait introduction and genetic uniformity.

Inventive Principle:
Principle #1Segmentation

2Reliability

If multiple desirable traits are combined in a single hybrid, then resistance to diseases and insects, heat and drought tolerance, and yield are improved, but maintaining uniformity of plant characteristics becomes more difficult

Engineering Contradiction:
Improveresistance to diseases and insects, heat and droughtVSAvoiduniformity of plant characteristics
Core Design Contradiction:
ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent combines multiple resistance traits and agronomic qualities into a single hybrid variety through systematic breeding. Inbred lines are developed with specific trait combinations, then crossed to produce uniform F1 hybrids that express all desired traits simultaneously while maintaining genetic uniformity through heterosis.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The breeding process optimizes specific genetic parameters including trait inheritance patterns, inbreeding coefficients, and hybrid vigor expression. By controlling these parameters through selective crossing and backcrossing, the patent achieves both multi-trait reliability and plant uniformity.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If locus conversion and transformation are used to introduce specific traits, then genetic integrity and enhanced performance are achieved, but the breeding process becomes more complex

Engineering Contradiction:
Improveyield and agronomic performanceVSAvoidbreeding process steps
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent performs preliminary development of inbred lines with desired traits through locus conversion before producing the final hybrid. This preliminary action ensures that the genetic foundation is established with optimal trait combinations, simplifying the subsequent hybrid production and reducing overall process complexity.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The breeding process incorporates feedback mechanisms including selection of progeny based on trait expression, evaluation of inbred line performance, and adjustment of crossing strategies. This feedback enables optimization of the complex breeding steps to achieve maximum productivity while managing process complexity.

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS9258962B1Maize variety hybrid X13B656
Publication Date: 2016.02.16 PIONEER HI BREED INTERNATIONAL INC
  • US9258962B1 patent drawing

AI summary

A novel maize variety designated X13B656 and seed, plants and plant parts thereof, produced by crossing Pioneer Hi-Bred International, Inc. proprietary inbred maize varieties. Methods for producing a maize plant that comprises crossing hybrid maize variety X13B656 with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13B656 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X13B656, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13B656. This invention further relates to methods for producing maize varieties derived from maize variety X13B656.