MALDI Matrix Observation Device UV Imaging
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Solution Overview
Problem
In conventional MALDI mass spectrometers, determining the optimal location for laser irradiation to achieve efficient ionization is challenging, as the location with suitable matrix state and intense sample signal may not coincide, and repeatedly acquiring measurement or mass spectral data is cumbersome.
Innovation Solution
A matrix observation device is employed, using a light source with a wavelength range overlapping the absorption range of the matrix, allowing precise observation of matrix distribution for efficient ionization, either through ultraviolet or infrared imaging, to identify the most effective irradiation location.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If visible light is used for observation, then the observation process is simple, but the matrix distribution cannot be precisely observed because visible light does not overlap with the matrix absorption range
Solution Approach 1:
The patent changes the wavelength parameter of the observation light from visible light to ultraviolet or infrared light, which overlaps with the matrix absorption range. This parameter change enables precise observation of matrix distribution while maintaining the simplicity of the observation process, as the system automatically selects the appropriate wavelength range based on the matrix type.
2Measurement precision
If repeatedly acquiring measurement or mass spectral data is performed to determine optimal irradiation location, then accurate ionization location can be found, but the analysis time increases significantly
Solution Approach 1:
The patent performs preliminary observation of matrix distribution using ultraviolet or infrared light before the actual mass spectrometry analysis. By identifying the optimal irradiation location in advance through this preliminary observation, the system avoids the need for repeated measurement acquisitions during the analysis phase, thereby significantly reducing analysis time while maintaining accurate ionization location determination.
3Device complexity
If the light source wavelength does not overlap with matrix absorption range, then the observation system is simpler, but the correlation between observed image and ionization efficiency is lost
Solution Approach 1:
The patent changes the wavelength parameter of the light source to overlap with the matrix absorption range. This ensures that the matrix absorbs the observation light, creating a visible correlation between the observed image and the actual ionization efficiency. The system maintains reliability by selecting wavelength ranges that match known matrix absorption characteristics.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables precise determination of the ionization-efficient location by correlating matrix distribution with ionization efficiency, simplifying the process and improving data accuracy in MALDI mass spectrometry.
Implementation Method 1
a sample containing a matrix that absorbs light having a particular wavelength range
Implementation Method 2
a light source unit for irradiating the sample plate with light for observation
Implementation Method 3
an image acquisition unit for detecting light from the sample plate so as to form an optical image
Implementation Method 4
a sample that has been mixed with a matrix is irradiated with a laser beam so as to gasify or ionize the sample
Implementation Method 5
part of the matrix that has absorbed the heat from the irradiation with the laser beam is rapidly heated
Data Source
AI summary
The invention provides a matrix observation device where a location to be irradiated with a laser beam that provides high efficiency of the ionization can be found from among sample spots arranged on a sample plate. The device is formed of: a stage 31 on which a sample plate 20 on which a sample is to be arranged is to be placed; a light source unit 40 that emits ultraviolet rays for observation with which the sample plate 20 is irradiated; and an image acquisition unit 50 for detecting light from the sample plate 20 so as to create an optical image, and the sample contains a matrix that absorbs the ultraviolet rays for observation.