Soluble Mannan-Oligosaccharide Extraction from Yeast Cell Walls
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Solution Overview
Problem
Current mannan-oligosaccharide (MOS) products in animal feed have limited differentiation and are mostly insoluble, which restricts their effectiveness in improving gastrointestinal health and overall performance in animals, and they require higher inclusion rates in feed, reducing space for essential nutrients.
Innovation Solution
A process involving alkali and acid hydrolysis to extract soluble mannan-oligosaccharides from yeast cell walls, followed by spray-drying to create a concentrated MOS product with enhanced MOS content, which is both solubilized and more effective in binding and removing pathogenic bacteria, thereby improving animal health and reducing feed inclusion rates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If simple cell wall products are used as MOS products, then manufacturing is easier and cost is lower, but MOS content is low and gastrointestinal protection effectiveness is limited
Solution Approach 1:
The yeast cell wall is segmented through selective extraction processes to separate and isolate mannan-oligosaccharides from other cell wall components. The process divides the complex cell wall structure into extractable MOS fractions and remaining cell wall material, enabling concentration of active ingredients while simplifying the final product composition for enhanced effectiveness.
Solution Approach 2:
Mannan-oligosaccharides are extracted from the yeast cell wall using alkaline treatment followed by acidification. This extraction process removes the active MOS components from the complex cell wall matrix, concentrating them in the soluble fraction while leaving behind insoluble cell wall material, thereby increasing MOS content in the final product.
2Reliability
If higher inclusion rates of MOS are used in feed, then gastrointestinal health improvement is achieved, but space for essential nutrients is reduced
Solution Approach 1:
The MOS product parameters are changed from simple cell wall material to concentrated, solubilized mannan-oligosaccharide extracts with enhanced molecular weight distribution. This parameter change increases the biological activity and effectiveness per unit, allowing lower inclusion rates in feed while maintaining or improving gastrointestinal protection effectiveness.
Solution Approach 2:
The final product combines concentrated MOS extracts with remaining cell wall components to create a composite material that maintains structural integrity while maximizing active ingredient content. This composite approach ensures both effectiveness and functional properties are preserved at optimized inclusion rates.
3Reliability
If soluble MOS is produced through extraction, then gastrointestinal protection is improved and feed inclusion rates can be reduced, but processing time and chemical usage increase
Solution Approach 1:
Alkaline treatment is applied preliminarily to the yeast cell wall to facilitate subsequent MOS extraction. This preliminary action weakens the cell wall structure and solubilizes mannan components, making the extraction process more efficient and reducing the overall time required for MOS liberation and concentration.
Solution Approach 2:
The extraction process uses periodic alternation between alkaline treatment and acidification steps. This periodic action enables controlled solubilization and precipitation cycles that efficiently concentrate MOS while managing processing time and chemical usage through repeated treatment stages.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process results in a 25-50% higher MOS content in the final product, providing superior gastrointestinal protection, increased active components, and a cost benefit by reducing feed inclusion rates, allowing more nutrients in the feed, while maintaining the integrity of yeast-derived components like β-glucan.
Implementation Method 1
A process involving alkali and acid hydrolysis to extract soluble mannan-oligosaccharides from yeast cell walls
Implementation Method 2
A process involving alkali and acid hydrolysis to extract soluble mannan-oligosaccharides from yeast cell walls
Implementation Method 3
followed by spray-drying to create a concentrated MOS product
Data Source
Figure 1
AI summary
The present invention relates to yeast cell wall 5 compositions enriched with mannose, mannose extracts and processes for making the same.