Mannanase Sequence Variants for Detergent pH and Stability Tradeoffs
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Solution Overview
Problem
Existing mannanases are not optimally suited for industrial applications, particularly in the detergent industry, requiring improved variants with enhanced stability and activity across a range of pH conditions.
Innovation Solution
Development of mannanase variants with specific modifications at defined positions, achieving at least 85-99% sequence identity to a parent polypeptide, which exhibit improved stability and activity in detergent compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing mannanases are used in detergent applications, then they can degrade mannan-containing stains, but they lack sufficient stability and activity across different pH conditions
Solution Approach 1:
The patent applies parameter changes by modifying specific amino acid residues at defined positions in the mannanase polypeptide sequence. These positional modifications alter the enzyme's physical and chemical properties, enabling it to maintain stability and activity across a broader pH range while preserving its core mannan-degrading function in detergent environments
2Reliability
If mannanase variants with multiple modifications are created, then stability and activity are improved, but the complexity of developing and characterizing variants increases
Solution Approach 1:
The patent applies local quality by focusing modifications on specific defined positions within the polypeptide sequence rather than random or global changes. This targeted approach at particular locations allows systematic improvement of stability and activity while maintaining a manageable framework for variant development and characterization
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified mannanase variants demonstrate enhanced stability and activity in detergent environments, effectively degrading mannan-containing stains and improving cleaning efficacy.
Implementation Method 1
The main enzyme type participating in the degradation of mannans are endo-1,4-β-mannanases (EC 3.2.1.78), which hydrolyze the internal glycoside bonds in the mannan backbone
Data Source
AI summary
The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and detergent compositions and methods of using the variants.