Mannanase Variants for Detergent Stability Across pH Ranges
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Solution Overview
Problem
Existing mannanases are not optimally suited for industrial applications, particularly in the detergent industry, due to limitations in stability and efficiency across varying pH ranges and environments.
Innovation Solution
Development of mannanase variants with specific amino acid modifications at defined positions, enhancing their stability and activity, allowing for improved performance in detergent compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing mannanases are used in detergent applications, then they can degrade mannan-containing stains, but they exhibit insufficient stability and efficiency across varying pH ranges and environmental conditions
Solution Approach 1:
The patent applies parameter changes by modifying specific amino acid residues at defined positions in the mannanase protein sequence. These sequence modifications alter the enzyme's physical and chemical properties, enabling it to maintain stability and activity across a broader pH range and in detergent environments, thus resolving the contradiction between reliability and adaptability
2Reliability
If mannanase variants with multiple amino acid modifications are developed, then stability and activity are enhanced, but the complexity of protein engineering increases
Solution Approach 1:
The patent applies local quality by focusing modifications on specific, predefined positions in the protein sequence rather than undertaking comprehensive protein engineering. By targeting particular residues that critically influence stability and activity, the approach enhances enzyme performance while limiting the scope of engineering complexity to manageable, localized changes
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified mannanase variants exhibit enhanced stability and activity, effectively degrading mannan-containing stains and improving cleaning efficacy in laundry detergents.
Implementation Method 1
The main enzyme type participating in the degradation of mannans are endo-1,4-β-mannanases (EC 3.2.1.78), which hydrolyze the internal glycoside bonds in the mannan backbone.
Data Source
AI summary
The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and detergent compositions and methods of using the variants.