Microbial Fermentation for Marine By-Product Fractionation

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

The processing of marine animal by-products, such as fish and shrimp, generates significant waste that is difficult to dispose of environmentally and economically, with existing methods like sun drying and chemical extraction causing hygienic issues and depolymerization of valuable compounds like chitin and astaxanthin.

Innovation Solution

A microbial composition comprising lactic acid bacteria and other microorganisms like Bacillus and Trichoderma is used for biodegradation, which breaks down marine animal by-products into solid, liquid, and lipid fractions, including chitosan and glucosamine, through fermentation, allowing for the separation and recovery of valuable compounds.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If chemical acids and alkalis are used for extraction, then chitin and protein hydrolysates can be recovered, but depolymerization and partial deacetylation of chitin occurs

Engineering Contradiction:
Improvechitin recoveryVSAvoidchitin polymer structure
Core Design Contradiction:
Quantity of substanceVSStability of the object's composition

Solution Approach 1:

The patent changes the extraction method from chemical (acids/alkalis) to biological (microbial fermentation), altering the extraction parameters to maintain chitin polymer integrity while achieving effective recovery. The microbial enzymes work under milder conditions that preserve the polymer structure.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces chemical extraction mechanisms with biological enzymatic mechanisms. Microbial enzymes naturally present in the fermentation process extract chitin without causing depolymerization, substituting harsh chemical actions with gentle biological actions.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Quantity of substance

If chemical methods are used for extraction, then protein hydrolysates can be recovered, but other products such as pigment recovery becomes complicated

Engineering Contradiction:
Improveprotein hydrolysate recoveryVSAvoidproduct separation process
Core Design Contradiction:
Quantity of substanceVSDevice complexity

Solution Approach 1:

The patent segments the extraction process into distinct microbial functions where different microorganisms target different components. This natural segmentation allows simultaneous recovery of multiple products (chitin, protein, pigment) through a single integrated fermentation process rather than multiple sequential chemical extractions.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The microbial consortium performs multiple functions simultaneously: chitin extraction by chitinolytic microorganisms, protein hydrolysis by proteolytic microorganisms, and pigment release. This multi-functional approach simplifies the overall process compared to separate chemical extraction steps for each component.

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Ease of manufacture

If sun drying is used for shrimp by-product utilization, then processing is simple, but hygienic control is poor and products are limited to animal consumption

Engineering Contradiction:
Improveprocessing simplicityVSAvoidhygienic control
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The fermentation process is self-regulating through microbial competition and pH control. Lactic acid bacteria lower the pH, creating an environment that prevents pathogenic bacterial growth, providing inherent hygienic control without requiring external monitoring or intervention.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent changes the processing method from physical drying to biological fermentation, altering the fundamental parameters of the process. This transformation maintains simplicity while improving hygiene through the natural antimicrobial properties of fermentation and produces higher-value products suitable for human consumption.

Inventive Principle:
Principle #35Parameter changes

4Ease of operation

If marine by-products are disposed of in landfills or dumped in ocean, then disposal is simple, but environmental problems and economic losses occur

Engineering Contradiction:
Improvedisposal simplicityVSAvoidenvironmental pollution
Core Design Contradiction:
Ease of operationVSObject-generated harmful factors

Solution Approach 1:

The patent converts the harmful waste material into beneficial products through fermentation. The same shrimp by-products that would cause pollution are transformed into valuable compounds (chitin, protein hydrolysates, pigments) that can be used in agriculture, food, and industrial applications, turning an environmental problem into an economic opportunity.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This process effectively converts marine by-products into valuable fractions like chitosan, glucosamine, and astaxanthin, improving the economic and environmental handling of waste while preserving nutritional quality and reducing environmental impact.

Implementation Method 1

A microbial composition comprising lactic acid bacteria and other microorganisms like Bacillus and Trichoderma is used for biodegradation, which breaks down marine animal by-products into solid, liquid, and lipid fractions

Methodology Applied
Scientific EffectFermentation: Fermentation

Implementation Method 2

biodegradation process and composition... microbial processes to treat marine animal by-products... breaks down marine animal by-products

Methodology Applied
Scientific EffectBiodegradation: Decomposition (biological)

Data Source

PatentEP2515674B1Biodegradation process and composition
Publication Date: 2016.03.09 AGRINOS
  • EP2515674B1 patent drawingFigure 1
  • EP2515674B1 patent drawingFigure 2

AI summary

Disclosed are novel microbial compositions and biodegradation processes to treat marine animal or marine animal by-products to produce solid, liquid and lipid fractions that contain useful compounds.