Mass Cytometry Sample Carriers for Soluble Analyte Quantitation
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current mass spectrometry techniques are limited in detecting and quantifying analytes in solution, as they cannot analyze analytes in solution effectively without immobilization, leading to challenges in multiparametric analysis and imaging.
Innovation Solution
The development of mass cytometry methods and sample carriers with surface modifications, such as polydopamine layers and 3D polymer brushes, enable the immobilization and quantitative analysis of soluble analytes on solid supports, allowing for precise quantitation and multiplexed analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If analytes in solution are analyzed directly by mass spectrometry, then analysis capability is maintained, but detection precision and quantitation accuracy deteriorate due to inability to immobilize analytes effectively
Solution Approach 1:
The patent introduces solid supports with surface modifications (polydopamine layers, 3D polymer brushes) as intermediaries to immobilize analytes from solution. These modifiers act as mediators that enable effective attachment of analytes to the solid phase, allowing mass spectrometry analysis with improved detection precision and quantitation accuracy while maintaining versatility in analyzing different analytes in solution
Solution Approach 2:
The patent modifies surface parameters of solid supports by introducing polydopamine coatings and 3D polymer brush structures. These parameter changes in surface chemistry and topology enhance the immobilization capability, enabling precise detection and quantitation of analytes that were previously difficult to analyze in solution phase
2Measurement precision
If analytes are immobilized on solid supports, then quantitation accuracy is improved, but device complexity increases due to surface modification requirements
Solution Approach 1:
The patent employs polydopamine surface modification as a versatile parameter change that can be applied to various solid support materials (glass, plastic, metal). This single modification approach simplifies the overall process by providing a universal platform for analyte immobilization across different device types, reducing the need for multiple specialized surface treatments
Solution Approach 2:
The patent creates composite structures by combining solid supports with polydopamine layers and 3D polymer brushes. These composite materials integrate the mechanical properties of the solid support with the functional properties of the surface modifiers, achieving high quantitation accuracy while maintaining relatively simple device architecture through material composition rather than structural complexity
3Quantity of substance
If surface modifications are added to sample carriers, then analyte immobilization capacity is improved, but manufacturing complexity increases
Solution Approach 1:
The patent uses polydopamine coating as a straightforward parameter change that can be applied through simple dip-coating or incubation processes. This approach dramatically increases analyte immobilization capacity by creating high-density attachment sites on the solid support surface, while the manufacturing process remains relatively simple and scalable
Solution Approach 2:
The patent performs preliminary surface modification with polydopamine and polymer brushes before analyte immobilization. This preliminary action prepares the surface with optimal binding properties in advance, enabling high immobilization capacity to be achieved through simple subsequent steps without requiring complex real-time manufacturing processes
4Productivity
If multiple analytes are analyzed simultaneously, then productivity is improved, but measurement precision deteriorates due to signal interference
Solution Approach 1:
The patent employs spatially resolved mass spectrometry imaging that analyzes multiple analytes simultaneously at different locations. By maintaining local quality of measurement at each pixel or region while building up a comprehensive map, the system achieves high productivity through multiplexed analysis without sacrificing measurement precision, as each analyte is detected with high accuracy at its specific location
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables precise quantitation of analytes in solution by immobilizing them on mass cytometry sample carriers, improving sensitivity and specificity, and allowing for the analysis of multiple analytes simultaneously, overcoming the limitations of existing techniques.
Implementation Method 1
surface modifications, such as polydopamine layers and 3D polymer brushes, enable the immobilization and quantitative analysis of soluble analytes on solid supports
Implementation Method 2
performing mass cytometry on the sample to determine the level of the one or more labelling atoms
Data Source
AI summary
Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.