Mass Spectrometer Intrascan Data Dependency Control
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Solution Overview
Problem
Current mass spectrometer systems lack the ability to automatically adjust scan parameters during a scan based on real-time data analysis, leading to inefficiencies and potential missed detection of marker ion ratios for peptides of interest.
Innovation Solution
A data-dependent method that allows for altering scan parameters, such as terminating or continuing a scan, based on user-specified criteria during the scan, using a controller to identify ion species of interest and adjust scanning resolutions accordingly within the mass spectrometer system.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If a scan is initiated in a mass spectrometer system, then mass spectral data is acquired, but the scan cannot be automatically terminated or continued based on real-time data analysis
Solution Approach 1:
The system continuously monitors mass spectral data during the scan and uses this feedback to dynamically决定是否继续或终止扫描。控制器根据实时检测到的离子物种信息调整扫描参数，实现了扫描过程的自适应控制
Solution Approach 2:
扫描参数从静态固定转变为动态可调，系统能够在扫描过程中根据数据依赖条件实时改变扫描分辨率、继续或终止扫描，使扫描过程具有动态适应性
2Productivity
If conventional data-dependent acquisition methods are used, then automated real-time decisions are made to maximize information content, but scan parameters cannot be altered during an initiated scan
Solution Approach 1:
系统可以根据实时数据判断只需采集部分扫描范围的数据即可满足分析需求，从而无需完成整个扫描周期。例如，当已检测到足够的标记离子比率信息时，提前终止扫描，避免了不必要的时间浪费
Solution Approach 2:
扫描系统能够自主根据实时数据分析结果决定是否继续扫描，无需外部干预。控制器自动评估数据质量并做出继续或终止扫描的决策，实现了系统的自我优化
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces overall cycle time, allows for more efficient analysis, and enables the examination of additional peptides that might otherwise be missed by making decisions based on acquired data during the scan.
Implementation Method 1
analyzer to separate such ionized ions by their masses by applying electric and magnetic fields
Implementation Method 2
a two-dimensional (2D) and/or a three-dimensional (3D) ion trap that enables the storage of ions over a large range of masses
Implementation Method 3
the use of an auxiliary voltage of low amplitude (e.g., up to about 20 volts at a duration of up to about tens of milliseconds) configured with a resonance frequency to match desired ions frequencies of motion
Implementation Method 4
as the amplitude increases, the kinetic energy of resonating ions also increases (i.e., as the square of the amplitude) and thus any collisions that occur with introduced neutral gas molecules or other ions become increasingly energetic. Eventually, the collisions which occur deposit enough energy into the molecular bonds of the resonating ions to cause bonds to break and thus cause fragmentation.
Data Source
AI summary
A mass spectrometer data dependent method and apparatus is introduced to alter scanning parameters based upon data acquired during that scan. Such a method an apparatus may include the identification of ion species of interest meeting user specified criteria so that a determination can be made as to whether or not the present scan is to be continued, terminated, or alternatively paused while such a decision is being made. Such a method of operation saves overall cycle time and allows examination of, for example, marker ion ratios for additional peptides that might otherwise be missed.