Megakaryocyte Compression Platelet Generation
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Solution Overview
Problem
Current methods for generating platelets using shear force are inefficient, resulting in low platelet production and poor functionality, and the prevailing understanding of platelet formation is believed to be incorrect by the inventors.
Innovation Solution
The method involves passing megakaryocytes or their precursors through a micro-passage system subjected to compression force, rather than shear force, to produce increased numbers of functional platelets or platelet-like particles.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If shear force is used to break up MK cells to generate platelets, then platelets are produced, but the production efficiency is low and platelet functionality is poor
Solution Approach 1:
The invention changes the physical parameter of force type from shear force to compression force. By applying compression force through micro-passages with diameters of 1-50 μm, the system achieves both high platelet production efficiency (1000-10000 platelets per MK cell) and maintains excellent platelet functionality, resolving the technical contradiction between productivity and reliability
Solution Approach 2:
The invention uses micro-passages with curved or rounded geometries rather than sharp edges. The micro-passages have smooth circular cross-sections with diameters optimized for cell deformation, allowing MK cells to undergo reversible deformation and generate platelets efficiently without damage, improving both production efficiency and platelet quality
2Productivity
If microporous membrane with MK cells squeezing through is used, then platelet generation is achieved, but only a few platelets are formed per MK cell
Solution Approach 1:
The invention employs micro-passages formed in porous materials or barriers with controlled pore sizes of 1-50 μm. These micro-passages allow MK cells to deform and pass through while generating platelets, achieving high productivity (1000-10000 platelets per MK cell) through the porous structure's ability to facilitate repeated cell passage
Solution Approach 2:
The system enables repeated periodic passage of MK cells through the micro-passages. MK cells can deform, pass through, recover, and pass through again multiple times (10-100 passages), with each passage generating platelets. This periodic action dramatically increases platelet yield per MK cell while maintaining a relatively simple device structure
3Productivity
If high flow liquid is used to shear off platelets from MK cells, then platelet generation occurs, but the platelets have poor functionality
Solution Approach 1:
The invention replaces the high-flow liquid shear force mechanism with a compression force mechanism. Instead of using high-velocity fluid flow to shear platelets, the system uses controlled compression through micro-passages, which generates platelets through cellular deformation and budding. This substitution produces platelets with superior functionality while maintaining high generation rates
Solution Approach 2:
The micro-passages act as an intermediary structure between the MK cells and the collection medium. Rather than direct shear force from high-flow liquid, the micro-passages mediate the platelet generation process by providing a controlled compression environment that preserves platelet functionality while enabling efficient generation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly increases platelet production and functionality, generating physiological levels of platelets with enhanced therapeutic properties, such as improved tissue repair and thrombotic activity, and is applicable for treating conditions like cancer, metabolic disorders, and ischemic diseases.
Implementation Method 1
Rather than using shear force to break up MK cells, the inventors have developed methods using compression force, particularly repeated compression force, applied to MK cells, resulting in platelet production.
Implementation Method 2
The step of passing the source material through the micro-passage particularly comprises or consists of passing one or more, especially a plurality of cells, through one or more micro-passages
Data Source
AI summary
The invention provides a method for generating platelets or platelet-like particles comprising passing a source material comprising one or more cells through a micro-passage to produce a passaged material comprising platelets or platelet-like particles. The invention also provides platelets and platelet-like particles produced using the method and uses thereof.