Megalocus Creation for Reliable Multi-Transgene Transmission
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Solution Overview
Problem
Current methods for introducing multiple transgenes into plants often face complications due to recombination, which can separate desired loci, making it difficult to reliably transmit multiple loci as a single genetic entity.
Innovation Solution
A method is developed to create a megalocus by obtaining hybrid plants with genetically linked transgenic loci, inducing genetic recombination, and selecting progeny with the loci arranged in cis, allowing for the stacking of multiple transgenes on the same chromosome, which can be introgressed into different plant varieties or species.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple transgenes are introduced into a plant using conventional methods, then the plant can possess multiple desired traits, but recombination can separate the desired loci making reliable transmission as a single genetic entity difficult
Solution Approach 1:
The patent merges multiple transgenic loci onto a single chromosome to form a megalocus, which segregates as a single genetic entity. This combining approach ensures that multiple desired traits are transmitted together through inheritance, resolving the recombination problem by physically linking the loci in cis configuration on the same chromosome.
Solution Approach 2:
The patent segments the chromosome by creating a megalocus that contains multiple transgenic loci arranged in cis. This segmentation allows the multiple loci to be treated as a single inheritable unit, separating them from other chromosomal regions and ensuring they are transmitted together as one genetic entity.
2Adaptability or versatility
If multiple transgenes are introduced into a plant, then the plant can exhibit multiple desired phenotypes, but extensive screening of progeny is required to identify plants with all desired traits
Solution Approach 1:
The patent performs preliminary action by constructing a megalocus that contains all desired transgenic loci in cis configuration before breeding. This pre-assembly ensures that all desired traits are linked together on a single chromosome, eliminating the need for extensive progeny screening to find plants with multiple traits, as they will be inherited together as a single unit.
3Stability of the object's composition
If transgenic loci are located on different homologous chromosomes, then each locus can be independently maintained, but recombination can separate desired loci
Solution Approach 1:
The patent merges loci that were previously on different homologous chromosomes onto a single chromosome in cis configuration. This merging creates a megalocus that is stably maintained as a single genetic entity, ensuring reliable co-transmission of all loci while maintaining their individual functional integrity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables the reliable transmission of multiple transgenes as a single genetic unit, reducing the complexity of trait integration and allowing for flexible testing and introgression of desired traits, such as enhanced growth, drought tolerance, and pest resistance, while avoiding the need for extensive screening of progeny.
Implementation Method 1
selecting a progeny plant produced from step (b) wherein genetic recombination has occurred between said first locus and said second locus to produce a megalocus comprising the first and second loci arranged in cis
Data Source
AI summary
The current invention provides methods for creating a block of genetically linked transgenic traits or a megalocus that can be transmitted as a single genetic unit. The present invention further provides methods for trait introgression to other plants, varieties or species using the megaloci of the invention. Also provided are plants, seeds, and plant parts comprising the megaloci.