Membrane Filter Deoxycholic Acid Surfactant Microorganism Recovery

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Solution Overview

Problem

Conventional membrane filters face challenges in maintaining precise pore size standards and achieving high recovery rates of microorganisms due to surfactant residues interfering with microbial growth and inadequate pore size for retaining small microorganisms like bacteria and mould spores.

Innovation Solution

The use of deoxycholic acid as a surfactant during membrane filter manufacturing, with a salt residue that minimizes interference with microbial growth, and orienting the air side facing the sample fluid to enhance microorganism retention, resulting in a recovery rate of 90% or better, along with a composition of cellulose acetate and cellulose nitrate and a grid pattern on the air side.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Manufacturing precision

If a conventional surfactant (sodium alkyl sulfonate) is used in the casting mix to ensure consistent filter quality and precise pore size, then manufacturing precision is improved, but the surfactant residue interferes with microbial growth, reducing recovery rate

Engineering Contradiction:
Improvepore size consistencyVSAvoidmicroorganism recovery rate
Core Design Contradiction:
Manufacturing precisionVSReliability

Solution Approach 1:

The patent changes the chemical parameter of the surfactant from sodium alkyl sulfonate to deoxycholic acid. This parameter change maintains the surfactant's function in producing consistent pore sizes while its salt residue (deoxycholate) has minimal interference with microbial growth, thereby resolving the contradiction between manufacturing precision and recovery rate

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent converts the previously harmful surfactant residue into a beneficial or neutral substance. Deoxycholic acid salt residue, while still present in the filter, does not significantly inhibit microbial growth unlike conventional surfactants. This transforms the harmful effect into an acceptable condition, maintaining both filter quality and high recovery rates

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

2Reliability

If the pore size is reduced to retain small microorganisms like bacteria and mould spores, then microorganism retention is improved, but fluid flow through the filter decreases

Engineering Contradiction:
Improvemicroorganism retentionVSAvoidfluid flow rate
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent applies local quality by orienting the air side of the membrane (which has optimal pore structure for retention) toward the sample fluid. This localized optimization allows the filter to achieve high retention of small microorganisms while maintaining adequate fluid flow, as the air side's structural characteristics are strategically positioned to face the fluid stream

Inventive Principle:
Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The deoxycholic acid surfactant and air-side orientation improve microorganism recovery rates significantly, achieving a 90% or better recovery rate and maintaining filter quality by reducing surfactant residue impact on microbial growth.

Implementation Method 1

The use of deoxycholic acid as a surfactant during membrane filter manufacturing, with a salt residue that minimizes interference with microbial growth

Methodology Applied
Scientific EffectSurfactant: Surfactant

Implementation Method 2

filtering a sample of the liquid to be analysed, optionally using differential pressure across the filter, to retain any microorganisms in the filter

Methodology Applied
Scientific EffectFiltration: Filter (physical)

Implementation Method 3

The nutrient medium stimulates the growth of any microorganisms present on the filter. After the predetermined time, any microorganisms caught in the filter should have grown into colonies which are visible to the naked eye

Methodology Applied
Scientific EffectMicrobial growth: Fermentation

Data Source

PatentEP2819772B1Membrane filter including bile acid and a method of manufacturing the same
Publication Date: 2024.03.20 GLOBAL LIFE SCI SOLUTIONS GERMANY GMBH
  • EP2819772B1 patent drawingFigure 1~2

AI summary

A membrane filter 26 is disclosed comprising cellulous material 23 allowing the transition of fluid therethrough, and, in a substantially dry state, said membrane comprising also a salt of deoxycholic acid. Optionally, the air side of the membrane (the side facing away from the screen or belt used to manufacture the membrane) faces the sample fluid during use of the membrane. A method of manufacture of the membrane material is disclosed also, employing deoxycholic acid as a surfactant, to improve the recovery rate of the membrane filter in use.