Porous Membrane Filtration Evaluation Under Suppressed Cell Growth
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Solution Overview
Problem
There is a need for an effective method to evaluate filtration conditions in continuous cell culture systems using porous membranes to separate cells and products efficiently, as existing methods struggle to maintain optimal cell growth environments over extended periods.
Innovation Solution
An evaluation method involving circulating cell broth between a culture vessel and a porous membrane under conditions that suppress cell proliferation, allowing for the assessment of filtration performance by returning unfiltered and filtered broth to the vessel, and evaluating conditions based on parameters such as temperature, enzyme activity, and membrane properties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If cells are cultured continuously with filtration to maintain high cell density and productivity, then product production efficiency is improved, but it becomes difficult to evaluate filtration conditions accurately because cells continue to proliferate
Solution Approach 1:
The invention performs preliminary action by suppressing cell proliferation before filtration evaluation. The cell broth is treated with a cell cycle inhibitor or cooled to below 15°C to stop cell division, ensuring that any changes in cell density or product concentration are solely due to filtration effects rather than ongoing cell growth. This allows accurate evaluation of filtration performance.
Solution Approach 2:
The invention segments the evaluation process into distinct phases: first suppressing cell proliferation, then performing filtration, and finally evaluating conditions. This segmentation allows independent assessment of filtration parameters without interference from concurrent cell proliferation, resolving the measurement precision issue while maintaining productivity through systematic evaluation.
2Reliability
If cell broth is circulated through porous membrane for filtration in continuous culture, then separation of cells from product is improved, but cell proliferation continues to complicate the evaluation of filtration performance
Solution Approach 1:
Before initiating the filtration evaluation, the invention first suppresses cell proliferation using cell cycle inhibitors or temperature control (below 15°C). This preliminary action simplifies the evaluation process by eliminating the confounding variable of ongoing cell division, making it easier to assess filtration performance without complex simultaneous control of multiple variables.
Solution Approach 2:
The invention employs feedback by monitoring cell density and product concentration changes during the filtration process. By suppressing proliferation beforehand, any observed changes can be directly attributed to filtration effects, providing clear feedback on membrane performance without the noise of concurrent cell growth.
3Productivity
If filtration is performed under normal cell proliferation conditions, then continuous production is maintained, but accurate assessment of membrane filtration performance cannot be achieved
Solution Approach 1:
The invention applies preliminary action by suppressing cell proliferation before filtration assessment. Cell cycle inhibitors or temperature reduction to below 15°C are used to halt cell division, ensuring that filtration performance can be accurately measured without the complicating factor of ongoing production activities.
Solution Approach 2:
The invention changes the temperature parameter to below 15°C or uses cell cycle inhibitors to alter cell behavior before filtration evaluation. This parameter change suppresses proliferation, allowing precise measurement of filtration performance while maintaining the ability to resume normal production by reversing the parameter change.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables accurate evaluation of filtration conditions, maintaining consistent cell density and product concentration, simplifying operations, and ensuring efficient product production by stabilizing cell growth and membrane performance.
Implementation Method 1
feeding a cell broth containing cells from a culture vessel containing the cell broth to a porous membrane
Data Source
AI summary
There is provided an evaluation method of a filtration condition, the evaluation method including, under a condition in which proliferation of cells is suppressed, feeding a cell broth containing cells from a culture vessel containing the cell broth to a porous membrane, returning a cell broth that has passed through the porous membrane without being filtered, to the culture vessel, and circulating the cell broth between the culture vessel and the porous membrane; and evaluating one or more filtration conditions with the porous membrane.