Blotting Membrane Spreader for Uniform Low-Volume Antibody Reaction
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Solution Overview
Problem
Conventional antibody reaction methods require large amounts of antibody solution, are cumbersome, and result in uneven distribution and inefficient antigen detection, especially with varying membrane sizes, orientations, and positions.
Innovation Solution
A CDR antibody reaction apparatus with a chamber part and a movable spreader that evenly distributes antibody solution on a blotting membrane, allowing for efficient antigen detection with a small amount of solution, regardless of membrane size or position, and facilitating easy solution addition/removal.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If a rotating cylindrical tube is used in conventional R-CDR method, then antibody solution can be circulated, but the reaction vessel must be removed and lid opened for each step to add or remove solution, complicating the use
Solution Approach 1:
The reaction vessel is segmented into a removable reaction vessel portion and a stationary base portion with drive mechanism. This allows the reaction vessel to be easily removed and reinstalled without affecting the drive mechanism, simplifying operation while maintaining automated rotation capability.
Solution Approach 2:
The drive mechanism is extracted from the reaction vessel itself and placed in a separate stationary base. This separation allows the reaction vessel to be freely removed and installed without carrying the drive mechanism, reducing operational complexity.
2Quantity of substance
If a small amount of antibody solution is used in rotating reaction vessel, then solution volume is reduced, but the antibody solution does not flow evenly due to wrinkles, hydrophilicity differences, and surface stains
Solution Approach 1:
A hydrophilic coating is applied as an intermediary layer on the reaction vessel inner surface. This coating mediates between the hydrophobic plastic surface and the antibody solution, ensuring uniform wetting and flow distribution even with small solution volumes, while the smooth coated surface prevents wrinkles and stains from affecting flow uniformity.
3Quantity of substance
If a large amount of antibody solution is used, then full immersion occurs, but the unique effect of CDR method disappears
Solution Approach 1:
The reaction vessel rotates dynamically during the antibody reaction process, creating cyclic draining and replenishing of the antibody solution. This dynamic motion ensures that even with limited solution volume, all areas of the membrane are uniformly exposed to fresh antibody solution, maintaining CDR effectiveness without requiring full immersion.
4Manufacturing precision
If conventional bath method is used with sufficient antibody solution to cover membrane, then complete coverage is achieved, but the process is time-consuming and requires large amount of solution
Solution Approach 1:
The reaction vessel undergoes periodic rotation during the antibody reaction, creating cyclic draining and replenishing of antibody solution across the membrane surface. This periodic action ensures complete coverage of the membrane with antibody solution over time, achieving uniform reaction without requiring a large initial solution volume.
Solution Approach 2:
The continuous rotation of the reaction vessel maintains continuous contact between the antibody solution and the membrane surface throughout the reaction period. This continuous useful action ensures complete and uniform coverage of the membrane, preventing any areas from being missed while using minimal solution volume.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables uniform antibody reaction and efficient antigen detection with reduced solution usage, suitable for automation, and improved signal quality and sensitivity compared to conventional methods.
Implementation Method 1
the position of the spreader moves along the top of the blotting membrane... the antibody solution may be evenly spread on the blotting membrane
Implementation Method 2
a chamber part in which an antibody solution is received... antibody solution in an amount sufficient to cover the lower part of the reaction vessel
Data Source
AI summary
A cyclic draining and replenishing (CDR) antibody reaction apparatus according to the present invention comprises: a chamber part inside which an antibody solution is accommodated, and in which a blotting membrane that reacts with the antibody solution is arranged; and a spreader arranged on the blotting membrane in the chamber part, wherein the position of the spreader moves along the top of the blotting membrane.