Merocyanine Dye for Stable Biomolecule Labeling
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Solution Overview
Problem
There is a scarcity of fluorescent dyes in biotechnology that exhibit low photobleaching, quenching, and high quantum efficiency, particularly in aqueous solutions and across various pH conditions, limiting their application in optical imaging and biomolecule detection.
Innovation Solution
A novel merocyanine-based compound with a specific chemical structure, capable of emitting a fluorescence signal at visible light wavelengths of at least 380 nm, is developed for use as a biomolecular labeling dye, kit, and contrast agent, which can be bonded to biomolecules to enhance imaging and detection capabilities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional fluorescent dyes (coumarin, cyanine, BODIPY, fluorescein, rhodamine, pyrene, carbopyronin, oxazine, xanthene, thioxanthene, or acridine skeletons) are used, then they have been widely used in various research areas, but they exhibit high photobleaching and quenching and low quantum efficiency in aqueous solutions and under various pH conditions
Solution Approach 1:
The patent changes the chemical structure parameters of fluorescent dyes by introducing a merocyanine skeleton with specific substituents (Ar1, Y1, Y2, Q, R1, Ra, R2-R4) to achieve improved quantum efficiency and stability in aqueous solutions across various pH conditions while maintaining low photobleaching and quenching
Solution Approach 2:
The patent creates composite fluorescent dye structures by combining the merocyanine core with various aromatic groups (Ar1), heteroatoms (Y1, Y2, Q), and substituent groups (R1, Ra, R2-R4) to achieve superior performance in quantum efficiency, stability, and resistance to photobleaching and quenching compared to conventional single-structure dyes
2Measurement precision
If fluorescent dyes are used to label biomolecules for optical imaging, then visualization capability is achieved, but the scarcity of dyes with low photobleaching, quenching, and high quantum efficiency limits application in aqueous solutions and across various pH conditions
Solution Approach 1:
The patent modifies the chemical parameters of fluorescent dyes through the merocyanine structure with adjustable substituents to achieve high quantum efficiency and stability in aqueous environments across different pH conditions, thereby expanding adaptability while maintaining detection precision
Solution Approach 2:
The patent designs fluorescent dyes with universal applicability by creating a merocyanine-based structure that functions effectively in aqueous solutions and maintains performance across various pH conditions, enabling broad use in different biological imaging applications
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The merocyanine-based compound effectively generates a fluorescence signal at visible light wavelengths, enabling its use as a biomolecular labeling dye, kit, and contrast agent, offering improved sensitivity and stability for biomolecule detection and imaging applications.
Implementation Method 1
a novel merocyanine-based compound which exhibits a fluorescence signal at a visible light region of at least 380 nm
Data Source
AI summary
The present invention relates to: a novel merocyanine-based compound which exhibits a fluorescence signal at a visible light region of at least 380 nm, and which may be used for detecting biomolecules; and a biomolecular labeling dye, kit and contrast agent composition comprising the same.