Mesenchymal Cell Population for Spontaneous Cell Sheet Detachment
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for detaching mesenchymal stem cells from culture substrates involve the use of chemical substances like trypsin, which can damage cells and require special culture substrates, increasing production costs.
Innovation Solution
A cell population comprising mesenchymal cells with specific CD marker profiles (CD324 ≥ 70%, CD90 ≥ 90%, CD326 ≤ 10%) that can spontaneously detach from substrates without chemical or physical means, allowing for efficient production and use in pharmaceutical compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If chemical means like trypsin are used to detach mesenchymal stem cells from culture substrate, then cells can be detached, but the cultured cells are damaged by chemical substances
Solution Approach 1:
The patent extracts and removes the harmful chemical detachment agents (trypsin, proteases) from the cell detachment process. Instead, it uses a special culture substrate with temperature-reactive polymer coating that enables physical detachment through temperature change alone, thereby eliminating chemical damage to the mesenchymal stem cells while achieving effective detachment
Solution Approach 2:
The patent replaces the chemical detachment mechanism with a physical/thermal mechanism. By using temperature-reactive polymers that change their cell-adhesive properties in response to temperature changes, the system substitutes chemical action with a controllable physical process, allowing gentle and reversible cell detachment without chemical damage
2Ease of operation
If chemical means like trypsin are used to detach mesenchymal stem cells from culture substrate, then cells can be detached, but the detached cells are separated to single cells and cannot be detached in the form of a cell sheet
Solution Approach 1:
The patent removes harmful chemical agents that cause cell separation and instead employs a physical detachment method using temperature-reactive polymers. This approach preserves the natural cell-cell adhesion forces and extracellular matrix structures, enabling cells to detach collectively as intact sheets rather than separating into individual cells
Solution Approach 2:
The patent changes the physical parameter of temperature to control the adhesive properties of the polymer coating. By lowering the temperature below the polymer's transition point, the coating becomes non-adhesive, allowing the entire cell sheet to detach gently as a unified structure without chemical disruption of cell-cell junctions
3Ease of operation
If a particular material as culture substrate is used to detach cells depending on temperature changes, then cells can be detached, but this increases production cost
Solution Approach 1:
The patent makes the culture substrate multi-functional by coating it with temperature-reactive polymers that provide both cell adhesion during culture and temperature-responsive detachment. This single substrate system replaces the need for separate chemical detachment reagents and special equipment, reducing overall production costs while maintaining the ability to detach cells as sheets through simple temperature control
Solution Approach 2:
The temperature-reactive polymer coating on the culture substrate provides self-service by automatically changing its adhesive properties in response to temperature changes. The substrate itself performs the detachment function without requiring external chemical agents or complex mechanical devices, simplifying the process and reducing costs
Data Source
AI summary
This invention provides a cell population comprising mesenchymal cells capable of forming a cell sheet that can be spontaneously detached from a substrate. In such cell population comprising mesenchymal cells, the proportion of cells positive for CD324 is 70% or more and the proportion of mesenchymal cells positive for CD90 is 90% or more.