Metal Oxide Filter with Non-Square Holes for Nucleated Cell Collection
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Solution Overview
Problem
The existing cell trapping filter materials have a limited collection rate for nucleated cells, which hinders efficient separation and processing in biological applications.
Innovation Solution
A filter with a metal or metal oxide base and through holes of non-square shapes, specifically designed with a smaller inscribed ellipse diameter than the nucleated cells, enhances the collection rate by reducing deformation and adhesion, and includes a frame with varying film thickness to form a concave surface for fluid reservoir formation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional cell trapping filter materials are used, then filtering can be performed, but the collection rate of nucleated cells is limited
Solution Approach 1:
The invention changes the physical parameters of the filter, specifically using a metal or metal oxide material with controlled surface roughness (arithmetic average roughness smaller than the nucleus size) and non-square through-hole shapes. These parameter changes enable the filter to achieve a collection rate of 90% or more for nucleated cells, resolving the contradiction between reliable cell collection and filtering efficiency.
2Reliability
If the filter material has high adhesion properties, then cell collection rate improves, but cell deformation and damage increase
Solution Approach 1:
The invention optimizes the surface roughness parameter of the filter material, setting the arithmetic average roughness to be smaller than the size of the cell nucleus. This parameter control allows the filter to achieve high collection rates while minimizing cell deformation and adhesion damage, as the smooth surface reduces mechanical stress on the cells during filtration.
Solution Approach 2:
The invention uses metal or metal oxide materials with specific surface properties that combine appropriate adhesion characteristics with low deformation effects. The metal/metal oxide composite structure provides both the necessary cell trapping capability and the gentle surface interaction that prevents cell damage.
3Reliability
If conventional filter materials are used, then filtering can be performed, but processing time is extended
Solution Approach 1:
The invention changes the material parameters to metal or metal oxide with controlled surface roughness and optimized through-hole geometry (non-square shapes). These parameter changes increase the filtration speed while maintaining high collection rates, thereby reducing the overall processing time and resolving the time loss issue.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The filter significantly improves the collection rate of nucleated cells, reduces processing time, and maintains cell activity by preventing elution of filter materials into the solution, while facilitating easy handling and separation.
Implementation Method 1
a filter body containing at least either a metal or a metal oxide as a main component thereof and defining a plurality of through holes therein, each of the plurality of through holes having a shape other than a square shape. An arithmetic average roughness of a first side of the filter part is smaller than a size of a nucleus of the nucleated cells to be filtered
Data Source
AI summary
A filter for filtering nucleated cells that includes a body containing at least either a metal or a metal oxide as its main component; and plural through holes, each of which have a shape other than a square shape, formed therein. An arithmetic average roughness of a first side of the filter part is smaller than a size of a nucleus of the nucleated cells to be filtered.