Metal-Regulated Pasteurella Proteins for Cross-Serotype Vaccine Protection
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Solution Overview
Problem
Current vaccines against Pasteurella multocida, which cause avian diseases like fowl cholera, do not provide cross-protection across different serotypes due to variations in iron-acquisition systems among bacterial strains, limiting their effectiveness in heterologous challenges.
Innovation Solution
Compositions comprising specific metal-regulated proteins from Pasteurella multocida, isolated under iron-restricted conditions, are used to vaccinate animals, providing heterologous protection against multiple serotypes by targeting common iron-acquisition mechanisms.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current vaccines against Pasteurella multocida are used, then protection against specific serotypes is achieved, but cross-protection across different serotypes is not provided
Solution Approach 1:
The vaccine composition uses metal-regulated proteins that are universally expressed across different Pasteurella multocida serotypes. These proteins perform the same protective function against multiple serotypes (1, 3, 4, and other heterologous strains) rather than being specific to a single serotype, achieving multi-functionality in vaccine protection
Solution Approach 2:
The invention changes the parameter of vaccine antigen selection from serotype-specific surface proteins to metal-regulated proteins that are conserved across serotypes. By targeting proteins regulated by metal ions (iron, zinc, copper) that are essential for bacterial survival across all serotypes, the vaccine achieves broad-spectrum protection while maintaining high reliability against challenge infections
2Reliability
If vaccines target serotype-specific antigens, then high protection against homologous strains is achieved, but heterologous protection against different serotypes is limited
Solution Approach 1:
The invention extracts and isolates specifically the metal-regulated proteins from Pasteurella multocida that are conserved across different serotypes. By taking out these specific proteins (encoded by genes such as fur, znuA, cueO) that are essential for bacterial metal acquisition and are universally present, the vaccine achieves both homologous and heterologous protection without being constrained by serotype-specific variations
Solution Approach 2:
The metal-regulated proteins serve as universal antigens that trigger immune responses effective against multiple serotypes. These proteins perform the dual function of being essential for bacterial survival (making them consistent targets) and being conserved across serotypes (enabling cross-protection), thus achieving universality in vaccine efficacy
Data Source
AI summary
The present invention provides isolated proteins isolatable from a Pasteurella spp. such as P. multocida. Also provided by the present invention are compositions that include one or more of the proteins, and methods for making and methods for using the proteins.