Novel metalloproteases
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Solution Overview
Problem
There is a need for novel proteases suitable for specific conditions and uses in industrial applications, as existing proteases do not adequately address the requirements for stability and effectiveness in detergent compositions, particularly in cleaning applications.
Innovation Solution
A composition comprising a polypeptide with at least 90% sequence identity to a specific amino acid sequence, derived from Bacillales or Planococcus species, combined with surfactants and other enzymes, which retains protease activity across a wide pH and temperature range, suitable for use in various cleaning detergents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing proteases are used in detergent compositions, then cleaning function is provided, but stability and effectiveness under specific detergent conditions are insufficient
Solution Approach 1:
The patent applies parameter changes by identifying and modifying specific amino acid residues in the protease sequence (e.g., positions 17, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100) to enhance stability and effectiveness under detergent conditions while maintaining proteolytic activity across a wide pH and temperature range
Solution Approach 2:
The patent achieves universality by developing a protease variant that functions effectively across multiple detergent types (laundry, dishwashing, hard-surface cleaning) and under varying conditions (pH 4.5-10, temperature 30°C-70°C), making it a versatile enzyme suitable for diverse cleaning applications
2Productivity
If protease activity is maintained across wide pH and temperature ranges, then cleaning effectiveness is improved, but enzyme stability becomes more challenging
Solution Approach 1:
The patent resolves this contradiction by making parameter changes to the enzyme's amino acid sequence, specifically modifying residues that affect the enzyme's conformational stability and active site architecture, enabling it to maintain both broad operational range and structural integrity under varying pH and temperature conditions
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polypeptide maintains significant protease activity between pH 4.5 and 10 and 30°C to 70°C, effectively hydrolyzing proteins in detergent compositions, enhancing cleaning performance in laundry, dishwashing, and hard-surface cleaning applications.
Implementation Method 1
a polypeptide comprising an amino acid sequence having at least 90% or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 33 and a surfactant, wherein said polypeptide has protease activity
Implementation Method 2
the polypeptide maintains significant protease activity between pH 4.5 and 10 and 30°C to 70°C, effectively hydrolyzing proteins in detergent compositions
Data Source
Figure 1.1~1.2
Figure 1.3~1.4
Figure 1.5A~1.5B
AI summary
Aspects of the present compositions and methods relate to novel metalloproteases, polynucleotides encoding the novel metalloproteases, and compositions and methods for use thereof.