Methanol Storage Composition for DNA Methylation Stability
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Solution Overview
Problem
DNA methylation patterns in biological samples are prone to deviation from original patterns when stored at room temperature for extended periods, necessitating improved storage conditions to preserve these patterns.
Innovation Solution
A composition comprising a biological sample with a methylated DNA sequence and a storage solution of methanol, which maintains methylation patterns for prolonged periods, even at room temperature, with varying concentrations of methanol and water.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If biological samples are stored at room temperature for extended periods, then storage convenience is improved, but methylation pattern stability deteriorates
Solution Approach 1:
The patent changes the chemical composition parameters of the storage medium by incorporating methanol at specific concentrations (10-100%) along with formic acid and acetic acid. This chemical parameter modification enables the storage solution to preserve methylation patterns at room temperature, resolving the contradiction between storage convenience and methylation stability without requiring specialized cold storage conditions.
2Device complexity
If biological samples are stored without special preservatives, then storage simplicity is improved, but methylation pattern preservation deteriorates
Solution Approach 1:
The patent introduces a specifically formulated storage solution containing methanol, formic acid, and acetic acid as an intermediary medium between the biological sample and the storage environment. This intermediary solution actively protects the methylation patterns from degradation while maintaining a simple liquid storage system, thus resolving the contradiction between storage simplicity and pattern preservation reliability.
Data Source
AI summary
The disclosure provides methods for storage solutions for preserving DNA methylation patterns over a period of time. The disclosure also provides for methods of using methylated DNA stored in such storage solutions.